中国药房2024，Vol.35Issue(17)：2108-2113,6.DOI:10.6039/j.issn.1001-0408.2024.17.09
术后通气方对术后肠麻痹小鼠肠道损伤的改善作用及机制
Improvement effect and mechanism of Shuhou tongqi formula on intestinal injury in mice with postoperative ileus
摘要
Abstract
OBJECTIVE To study the improvement effect and mechanism of Shuhou tongqi formula on intestinal injury in mice with postoperative ileus(POI).METHODS Mice were randomly divided into sham operation group,model group,positive control group(Mosapride citrate tablets,1.95 mg/kg),and Shuhou tongqi formula group(1.88 g/kg),with 6 mice in each group.Except for the sham operation group,POI model was induced in other groups by typical small intestinal interference.Each group was given relevant drug liquid/water,once a day,for consecutive 2 days.After the last medication,the percentage of carbon powder propulsion in small intestine was detected,and pathomorphological changes in ileum tissue of mice were observed.The serum levels of interleukin-6(IL-6),IL-10,tumor necrosis factor-α(TNF-α),motilin(MTL)and somatostatin(SS)were all detected;the expression levels of Toll-like receptor 4(TLR4),nuclear factor κB(NF-κB p65)and p38 mitogen-activated protein kinase(p38 MAPK)were determined in ileal tissue of mice;the gut microbiota of colon contents was analyzed in each group of mice.RESULTS After the intervention of Shuhou tongqi formula,pathological damage such as intestinal wall atrophy and mucosal capillary congestion in ileum tissue were improved significantly;the percentage of carbon powder propulsion and the serum level of IL-10 and MTL were increased significantly(P＜0.05);however,serum levels of TNF-α,IL-6 and SS,the expressions of TLR4,NF-κB p65 and p38 MAPK were decreased significantly(P＜0.05).The analysis of gut microbiota showed that Shuhou tongqi formula could significantly increase ACE,Chao1,Shannon and PD indexes,and relative abundance of Akkermansia(P＜0.05),but decreased relative abundance of Proteobacteria,Ligilactobacillus and Escherichia-Shigella significantly(P＜0.05).CONCLUSIONS Shuhou tongqi formula can improve intestinal injury and inflammatory reaction of POI mice,the mechanism of which may be associated with inhibiting the activity of TLR4/NF-κB/MAPK signaling pathway,regulating the levels of gastrointestinal hormones and improving the disturbance of intestinal flora in mice.关键词
术后通气方/术后肠麻痹/肠道损伤/炎症因子/肠道菌群Key words
Shuhou tongqi formula/postoperative ileus/intestinal injury/inflammatory factor/intestinal flora分类
药学引用本文复制引用
卢雨暄,江志伟,谢怡君,王弈竹,哈静文,束雅春..术后通气方对术后肠麻痹小鼠肠道损伤的改善作用及机制[J].中国药房,2024,35(17):2108-2113,6.基金项目
江苏省知识产权高价值专利培育计划项目(No.GJ20231160_01) （No.GJ20231160_01）
南京市医疗机构中药传统制剂研究立项项目(No.NJCC-ZJ-202303) （No.NJCC-ZJ-202303）
南京市知识产权项目 （）
