中国药理学通报2024，Vol.40Issue(9)：1665-1673,9.DOI:10.12360/CPB202404090
独活寄生汤对TNF-α诱导的类风湿性关节炎成纤维样滑膜细胞增殖、凋亡和炎症的影响
Effects of Duhuo Jisheng Decoction on TNF-α-induced proliferation,apoptosis and inflammation of fibroblast-like synoviocytes in rheumatoid arthritis
摘要
Abstract
Aim To explore the effect of serum contai-ning Duhuo Jisheng Decoction on the proliferation,ap-optosis and inflammation of fibroblast-like synoviocytes(FLS)induced by tumor necrosis factor-α(TNF-α)in rheumatoid arthritis(RA)and to reveal the under-lying mechanism.Methods The MH7A cells were divided into five groups:normal group(10％blank se-rum),model group(10 μg·L-1 TNF-α+10％blank serum),Duhuo Jisheng Decoction low(10 μg·L-1 TNF-α+2.5％drug-containing serum+7.5％blank serum),medium(10 pg·L-1 TNF-α+5％drug-con-taining serum+5％blank serum),high(10 μg·L-1 TNF-α+10％drug-containing serum)dose group.The concentration of serum containing Duhuo Jisheng Decoction was screened by MTT method.Cell prolifer-ation was detected using EdU staining.The expression of proliferation marker Ki67 was detected using immu-nofluorescence staining.The apoptosis rate was meas-ured by flow cytometry.The contents of IFN-γ,IL-4,IL-6 and IL-10 in each group were detected by ELISA.The mRNA and protein expression of Bax,Bcl-2,caspase-3 and TLR4/MyD88/NF-κB signaling pathway were detected by RT-qPCR and Western blot.Results Compared with the normal group,the cell prolifera-tion activity of the model group significantly increased,and the level of apoptosis decreased.The content of IFN-γ and IL-6 increased,and the content of IL-4 and IL-10 decreased.The TLR4/MyD88/NF-κB signaling pathway was activated.After the intervention of low,medium and high dose groups of serum containing Du-huo Jisheng Decoction,it could effectively improve the abnormal proliferation of cells and enhance apoptosis.At the same time,it inhibited the inflammatory re-sponse and the activation of TLR4/MyD88/NF-κB sig-naling pathway.Conclusions The serum containing Duhuo Jisheng Decoction can inhibit the abnormal pro-liferation of RA-FLS induced by TNF-α and the secre-tion of pro-inflammatory factors,and enhance apoptosis and anti-inflammatory levels.The mechanism may be related to the regulation of TLR4/MyD88/NF-κB sig-naling pathway.关键词
独活寄生汤/类风湿性关节炎/成纤维样滑膜细胞/增殖/凋亡/炎症因子/TLR4/MyD88/NF-κB信号通路Key words
Duhuo Jisheng Decoction/rheumatoid ar-thritis/fibroblast-like synoviocytes/proliferation/ap-optosis/inflammatory factors/TLR4/MyD88/NF-κB signaling pathway分类
医药卫生引用本文复制引用
吴婧若,步亚男,岳进茹,陈佳,王桂宇,张育敏..独活寄生汤对TNF-α诱导的类风湿性关节炎成纤维样滑膜细胞增殖、凋亡和炎症的影响[J].中国药理学通报,2024,40(9):1665-1673,9.基金项目
国家自然科学基金资助项目(No 82274365) （No 82274365）
山西省自然科学基金资助项目(No 20210302123301) （No 20210302123301）
山西省中医药科研基金资助项目(No 2023ZYYA031) （No 2023ZYYA031）
山西中医药大学"青年科学家培育专题"项目(No 2021PY-QN-01) （No 2021PY-QN-01）
