中国药理学通报2024，Vol.40Issue(9)：1673-1679,7.DOI:10.12360/CPB202404031
多疏蛋白CBX4通过p38 MAPK信号通路调控食管鳞癌细胞增殖与凋亡
CBX4 regulates proliferation and apoptosis of esophageal squamous cell carcinoma through p38 MAPK signaling pathway
摘要
Abstract
Aim To investigate the expression level of CBX4 in esophageal squamous cell carcinoma(ESCC)and the effect of CBX4 on ESCC proliferation and un-derlying molecular mechanisms.Methods The ex-pression of CBX4 in different cancers was analyzed in Pan-cancers.The expression level of CBX4 in ESCC was analyzed by t-test based on Gene Expression Omni-bus(GEO)data.The viability of CBX4-overex-pressed/knockdown ESCC cells was detected by MTT assay,colony formation assay and flow cytometry assay.Furthermore,the tumor volumn,tumor weight and Ki67 expression were measured by mouse xenograft assay and immunohistochemistry.The mRNA and protein ex-pression levels of apoptosis-related genes PARP、Bcl-2、Bax were determined by qRT-PCR and Western blot,respectively.In addition,the underlying molecular mechanism of CBX4 in ESCC was revealed by qRT-PCR and Western blot.Results CBX4 was upregulat-ed in various cancers.The expression level of CBX4 in ESCC was higher than that in normal tissues(P＜0.05)based on Gene Expression Omnibus(GEO)da-ta.Compared with the normal group,the proliferation of CBX4 knockdown ESCC cells was significantly in-hibited and the apoptosis was promoted(P＜0.05).Meanwhile,the mRNA and protein expression levels of cleaved PARP and Bax were upregulated while that of Bcl-2 was downregulated.In CBX4 overexpression group,tumor volume in vivo increased(P＜0.05).Immunohistochemical results also showed an increase in Ki67 expression.Furthermore,the results of RNA-seq,bioinformatics analysis and qRT-PCR experiments indicated that CBX4 probably regulated the prolifera-tion and apoptosis of ESCC through p38 MAPK signa-ling pathway.Conclusion CBX4 is highly expressed in ESCC and plays as an oncogene role,which might regulate cell proliferation through the p38 MAPK signa-ling pathway.关键词
食管鳞癌/CBX4/增殖/凋亡/p38 MAPK信号通路/药物靶点Key words
esophageal squamous cell carcinoma/CBX4/proliferation/apoptosis/p38 MAPK signaling pathway/drug target分类
医药卫生引用本文复制引用
马燕春,花雨艳,刘蕊,毋阿婧,尹晓洁,杨洁..多疏蛋白CBX4通过p38 MAPK信号通路调控食管鳞癌细胞增殖与凋亡[J].中国药理学通报,2024,40(9):1673-1679,7.基金项目
国家自然科学基金资助项目(No 81802440) （No 81802440）
山西省基础研究计划自然科学研究面上项目(No 2022030212211187) （No 2022030212211187）
