中国药理学通报2024，Vol.40Issue(9)：1695-1700,6.DOI:10.12360/CPB202401031
香兰素下调cGAS/STING信号通路改善肝内胆汁淤积大鼠肝组织损伤
Vanillin down-regulates cGAS/STING signaling pathway to improve liver tissue injury in rats with intrahepatic cholestasis
摘要
Abstract
Aim To investigate the effect of vanillin on the regulation of cyclic guanylate adenylate synthetase(cGAS)/stimulator of interferon gene(STING)signa-ling pathway on hepatic tissue injury in rats with intra-hepatic cholestasis(IC).Methods SD rats were randomly divided into normal group,IC group,vanillin group,cGAS overexpression group,and vanillin+cGAS overexpression group,with continuous adminis-tration for seven days.The body weight,liver weight and liver to body weight ratio of rats were measured.Liver function(ALT,AST,ALP,LDH),IC(TBIL,TBA)and liver fibrosis(HA,LN,PC Ⅲ)index were determined by ELISA.Liver pathology and fibrosis were observed using HE and Masson staining,and col-lagen volume fraction was calculated.The expression of cGAS/STING pathway related proteins in liver tissue was detected by Western blot.Results Vanillin could improve liver pathology and fibrosis,increase body weight,and decrease liver weight,ALT,AST,ALP,LDH,TBIL,TBA,HA,LN,PC Ⅲ,collagen volume fraction,cGAS,STING protein in IC rats(P＜0.05).Overexpression of cGAS could reverse the effects of vanillin on the above indicators in IC rats(P＜0.05).Conclusions Vanillin may improve liver function,IC,liver fibrosis,and liver tissue damage in IC rats by downregulating the cGAS/STING signaling pathway.关键词
香兰素/环鸟苷酸腺苷酸合成酶/干扰素基因刺激因子/肝内胆汁淤积/肝损伤/肝纤维化Key words
vanillin/cyclic guanylate adenylate syn-thetase/stimulator of interferon gene/intrahepatic cho-lestasis/liver injury/liver fibrosis引用本文复制引用
姜宁,蒲兰香,黄峰,王艳,裴鑫,宋均亚,张恩胜..香兰素下调cGAS/STING信号通路改善肝内胆汁淤积大鼠肝组织损伤[J].中国药理学通报,2024,40(9):1695-1700,6.基金项目
山东省医药卫生科技发展计划项目(No 2022WS119) （No 2022WS119）
