中国药理学通报2024，Vol.40Issue(9)：1708-1718,11.DOI:10.12360/CPB202403025
急性脑缺血/再灌注后小鼠皮层和海马部位神经细胞损伤的动态表征
Dynamic characterization of neuronal injury in cortex and hippocampus of mice after acute cerebral ischemia/reperfusion
摘要
Abstract
Aim To dynamically characterize neuronal damage in the cortex and hippocampus of mice follow-ing acute cerebral ischemia/reperfusion(I/R).Meth-ods Male C57BL/6J mice weighing 25-28 g under-went middle cerebral artery occlusion using the fila-ment method,followed by 1 hour of reperfusion to es-tablish the acute cerebral I/R injury mouse model.The experiment comprised a sham surgery group,I/R-6 h group,I/R-24 h group,and I/R-72 h group.Longa neurological function score was used to assess the neu-rological function.Triphenyltetrazolium chloride(TTC)staining was conducted to detect cerebral in-farct volume.Hematoxylin and eosin(HE)staining was utilized to observe brain tissue pathological dam-age.Nissl staining was performed to evaluate neuronal damage.Immunofluorescence histochemistry staining was employed to assess the activation of astrocytes and microglia,as well as neuronal loss.Transmission elec-tron microscopy was used to examine mitochondrial damage in hippocampal neurons.Western blot analysis was conducted to detect the expression levels of mito-chondrial fission-fusion-related proteins p-Drp1/Drp1,Mff,Fis1,and OPA1.Results With prolonged cere-bral I/R time,neurological functional impairment,cerebral infarct volume,neuronal damage in the cortex and hippocampus,glial cell activation,neuronal loss,and mitochondrial damage gradually worsened in mice.The expression of mitochondrial fission-related proteins increased gradually,while the expression of mitochon-drial fusion-related proteins decreased gradually.Con-clusions Neuronal pathological damage,such as glial cell activation,neuronal loss,and mitochondrial dam-age,is gradually aggravated with prolonged cerebral I/R time,which may be associated with mitochondrial dynamics imbalance.关键词
缺血/再灌注损伤/动态表征/神经元/星形胶质细胞/小胶质细胞/线粒体Key words
cerebral ischemia/reperfusion injury/dy-namic characterization/neurons/astrocytes/micro-glia/mitochondria分类
基础医学引用本文复制引用
李彤,白家鸣,施懿峻,闻彩名,崔琳,杨静娴,肖洪贺..急性脑缺血/再灌注后小鼠皮层和海马部位神经细胞损伤的动态表征[J].中国药理学通报,2024,40(9):1708-1718,11.基金项目
国家自然科学基金资助项目(No 82204648) （No 82204648）
辽宁省自然科学基金资助项目(No 2021-MS-250) （No 2021-MS-250）
辽宁中医药大学中医脏象理论及应用国家教育部重点实验室开放基金资助项目(No zyzx2105) （No zyzx2105）
辽宁中医药大学自然科学基金资助项目(No 2021LZY046) （No 2021LZY046）
