中国癌症防治杂志2024，Vol.16Issue(4)：405-411,7.DOI:10.3969/j.issn.1674-5671.2024.04.04
PPP2R5C对多发性骨髓瘤细胞增殖、凋亡和药物敏感性的影响
Effects of PPP2R5C on proliferation,apoptosis,and drug sensitivity of multiple myeloma cells
摘要
Abstract
Objective To investigate the effects of protein phosphatase 2 regulatory subunit B'gamma(PPP2R5C)on the proliferation,apoptosis and drug sensitivity of multiple myeloma(MM)cells.Methods The qRT-PCR and Western blot were used to detect the expression of PPP2R5C in human multiple myeloma cell lines(MM1S and RPMI-8226 cell lines)and normal peripheral blood mononuclear cells(PBMC).The MM 1S cells were transfected with PPP2R5C interfering siRNA(si-PPP2R5C group)and its negative control(si-CTRL group),and PPP2R5C overexpressing plasmid(OE-PPP2R5C group)and its negative control(OE-CTRL group),respectively.Cell proliferation was detected by CCK-8 method,and cell apoptosis was detected by flow cytometry.The cells in the si-PPP2R5C group and si-CTRL group were treated with different concentrations of Bortezomib(BTZ)for 24 h,cell viability was measured by CCK-8 method and half maximal inhibitory concentration(IC50)values were calculated.After 1 nmol/L BTZ intervention for 24 h,the expression levels of BCL-2 and BAX in the si-PPP2R5C and si-CTRL groups were detected by qRT-PCR and Western blot,and Caspase-3/7 activity was detected by Caspase 3/7 activity detection kit,and cell apoptosis was detected by flow cytometry.Results Compared with PBMC cells,PPP2R5C was highly expressed in MM 1S and RPMI-8226 cells at both mRNA and protein levels(all P＜0.001).Compared with the si-CTRL group,the cell proliferation activity of si-PPP2R5C group was inhibited after 48 h and 72 h of cell culture(all P＜0.001),the apoptosis rate was significantly increased(5.97％vs 14.39％,P＜0.001).Compared with the OE-CTRL group,the cell proliferation activity of OE-PPP2R5C group was enhanced at these time points after 48 h and 72 h of cell culture(all P＜0.01).The IC50 value of BTZ in the si-PPP2R5C group was significantly lower than that in the si-CTRL group(3.40 nmol/L vs 10.37 nmol/L,P＜0.001).Moreover,compared with the control group and si-CTRL+BTZ group,mRNA and protein level of BCL-2 in the si-PPP2R5C+BTZ group were decreased,while the mRNA and protein level of BAX,Caspase 3/7 activity and cell apoptosis rate were increased(all P＜0.05).Conclusions PPP2R5C is highly expressed in MM cell lines.PPP2R5C knockdown can inhibit the cell proliferation and promote apoptosis of MM cells,and increase the drug sensitivity of BTZ.关键词
多发性骨髓瘤/PPP2R5C/增殖/凋亡/耐药Key words
Multiple myeloma/PPP2R5C/Proliferation/Apoptosis/Drug resistance分类
医药卫生引用本文复制引用
梁远征,王赫男,王亮..PPP2R5C对多发性骨髓瘤细胞增殖、凋亡和药物敏感性的影响[J].中国癌症防治杂志,2024,16(4):405-411,7.基金项目
国家自然科学基金面上项目(82170181) （82170181）
北京市医师科学家培养计划(BJPSTP-2024-01) （BJPSTP-2024-01）
