中国肿瘤生物治疗杂志2024，Vol.31Issue(8)：786-795,10.DOI:10.3872/j.issn.1007-385x.2024.08.006
过氧化物酶体膜蛋白4通过细胞外信号调节激酶1/2信号通路促进肝细胞癌细胞的上皮间质转化
Peroxisomal membrane protein 4 promotes epithelial-mesenchymal transition in hepatocellular carcinoma cells through the ERK1/2 signaling pathway
摘要
Abstract
Objective:To investigate the impact of peroxisomal membrane protein 4(PXMP4)on the migration,invasion,and epithelial-mesenchymal transition(EMT)of hepatocellular carcinoma(HCC)cells.Methods:A total of 38 pairs of HCC and paracancerous tissue samples were collected from the Department of Pathology,the Third Affiliated Hospital of Xinxiang Medical College from January 2018 to December 2022.Bioinformatics and immunohistochemistry were used to analyze PXMP4 expression in HCC tissues and its correlation with clinicopathological features of HCC patients.PXMP4 was silenced in HCCLM3 and MHCC97H cells and overexpressed in Huh7 and MHCC97L cells.The silencing/overexpression efficiency was verified by WB assay and qPCR.CCK-8 assay,wound healing assay,and Transwell invasion assay were used to detect the effects of PXMP4 interference or overexpression on the proliferation,migration,and invasion abilities of HCC cells.WB assay was used to detect the protein expression of N-cadherin,E-cadherin,vimentin,extracellular signal-regulated kinase(ERK),and p-ERK in HCC cells with PXMP4 interference/overexpression or 0,5,10,and 20 μmol/L U0126 treatment.Results:Bioinformatics and immunohistochemistry showed that PXMP4 was highly expressed in HCC tissues(P<0.05).Clinicopathological analysis revealed that PXMP4 expression was correlated with the degree of tumor differentiation(P<0.05).In HCC cells,the silence of PXMP4 inhibited cell proliferation,invasion,and EMT process(all P<0.05),while overexpression of PXMP4 promoted cell proliferation,invasion,and EMT(all P<0.05).Furthermore,PXMP4 promoted EMT in HCC cells by activating the ERK1/2 signaling pathway,which was inhibited by U0126 treatment.Conclusion:PXMP4 is highly expressed in HCC tissues and is closely related to HCC cell differentiation.PXMP4 promotes EMT in HCC cells by activating the ERK1/2 signaling pathway.关键词
肝细胞癌/过氧化物酶体膜蛋白4/细胞外信号调节激酶/迁移/侵袭/上皮间质转化Key words
hepatocellular carcinoma(HCC)/peroxisomal membrane protein 4(PXMP4)/extracellular signal-regulated kinase(ERK)/migration/invasion/epithelial-mesenchymal transition(EMT)分类
医药卫生引用本文复制引用
李巍,徐梓山,马柯,张静雨,胡晓云,贺国洋..过氧化物酶体膜蛋白4通过细胞外信号调节激酶1/2信号通路促进肝细胞癌细胞的上皮间质转化[J].中国肿瘤生物治疗杂志,2024,31(8):786-795,10.基金项目
河南省高等学校重点科研项目(No.23B310002) （No.23B310002）
河南省大学生创新创业训练计划(No.202310472007) （No.202310472007）
