中国肿瘤生物治疗杂志2024，Vol.31Issue(8)：796-802,7.DOI:10.3872/j.issn.1007-385x.2024.08.007
落新妇苷通过调节HIF-1α/VEGF轴抑制乳腺癌细胞的增殖、迁移和血管生成拟态形成
Astilbin inhibits proliferation,migration and vasculogenic mimicry formation of breast cancer cells by regulating HIF-1α/VEGF axis
摘要
Abstract
Objective:To explore the effects of astilbin(AST)on the proliferation,migration and vasculogenic mimicry(VM)formation in breast cancer cells by regulating the hypoxia-inducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)axis.Methods:Breast cancer cells(MCF-7 and MDA-MB-231)were treated with AST at different concentrations(0,5,25,50,100,150,200,300 μmol/L),and the cell viability was detected using the CKK-8 assay.The MCF-7 and MDA-MB-231 cells were divided into control group,low-dose AST(AST-L),medium-dose AST(AST-M),high-dose AST(AST-H),and AST-H+DMOG(HIF-1α/VEGF pathway activator)groups.The effect of AST on cell proliferation was detected by EdU assay,the influence on apoptosis was detected using flow cytometry,the migration and invasion abilities of cells were examined using Transwell chamber assay,and the VM formation was analyzed using Matrigel tube formation assay.The expression of HIF-1α,VEGF,VE-cadherin,E-cadherin,N-cadherin and MMP-2 was detected by WB assay.Results:Compared with 0 μmol/L AST,the viability of cells treated with 5,25,50,100,150,200,and 300 μmol/L AST decreased obviously,which was in a dose-dependent manner(P<0.05).Compared with the control group,the AST-L,AST-M,and AST-H groups showed a significant decrease in EdU positive rate,cell migration number,cell invasion number,VM lumen number,and the protein expression of HIF-1α,VEGF,VE-cadherin,N-cadherin,and MMP-2,while the apoptosis rate and E-cadherin protein expression obviously increased(all P<0.05).Compared with the AST-H group,the AST-H+DMOG group demonstrated a significant increase in EdU positive rate,cell migration number,cell invasion number,VM lumen number,and the protein expression of HIF-1α,VEGF,VE-cadherin,N-cadherin,and MMP-2,while the apoptosis rate and E-cadherin protein expression significantly decreased(all P<0.05).Conclusion:AST can inhibit the proliferation,migration,invasion,and VM formation of breast cancer cells and promote apoptosis,which may be related to the inhibition of the HIF-1α/VEGF signaling pathway.关键词
乳腺癌/落新妇苷/增殖/迁移/血管生成拟态/低氧诱导因子-1α/血管内皮生长因子Key words
breast cancer/astilbin/proliferation/migration/vasculogenic mimicry(VM)/hypoxia-inducible factor-1α/vascular endothelial growth factor(HIF-1α/VEGF)分类
医药卫生引用本文复制引用
王媛媛,顾玉,刘秋霞,马胜辉,龚志平..落新妇苷通过调节HIF-1α/VEGF轴抑制乳腺癌细胞的增殖、迁移和血管生成拟态形成[J].中国肿瘤生物治疗杂志,2024,31(8):796-802,7.基金项目
承德市基础研究项目(No.202301A111) （No.202301A111）
