中国中医药信息杂志2024，Vol.31Issue(9)：41-47,7.DOI:10.19879/j.cnki.1005-5304.202309117
基于网络药理学和动物实验探讨达原饮治疗幽门螺杆菌阳性口周痤疮作用机制
Discussion on the Mechanism of Dayuan Decoction in the Treatment of Helicobacter pylori-Positive Perioral Acne Based on Network Pharmacology and Animal Experiments
摘要
Abstract
Objective To investigate the mechanism of Dayuan Decoction in the treatment of Helicobacter pylori(Hp)-positive perioral acne based on network pharmacology and animal experiments.Methods The effective active components and their corresponding targets of Dayuan Decoction were obtained from TCMSP.Targets related to Hp and perioral acne were retrieved from GeneCards,OMIM and TTD databases,and the intersection of drug targets and disease targets was considered as the target of Dayuan Decoction in the treatment of Hp-positive perioral acne.A protein-protein interaction network for the drug-disease intersection targets was constructed by using Cytoscape 3.7.2 and the STRING database,and the core targets were screened.GO and KEGG pathway enrichment analyses of the core target genes were performed using the DAVID database and the bioinformatics platform.By establishing an Hp-positive perioral acne rat model through gastric gavage with Hp bacterial liquid and intradermal injection of Propionibacterium acnes suspension,the intervention effect of Dayuan Decoction was observed and the core targets and related pathways were preliminary validated.Morphology in the perioral skin and gastric tissue were observed through HE staining.The expressions of TLR4,MyD88,NF-κB in perioral skin tissue,the contents of TNF-α and IL-6 in serum were measured using ELISA.Results A total of 145 active components were screened from Dayuan Decoction,corresponding to 253 targets,with 57 targets overlapping between drugs and diseases.The core targets for the treatment of Hp-positive perioral acne with Dayuan Decoction were identified as IL6,TNF,IL1B,AKT1,TP53,etc.KEGG pathway enrichment analysis revealed 153 signaling pathways,primarily involved in pathways in cancer,lipids and atherosclerosis,AGE-RAGE signaling pathway in diabetic complications,TLR signaling pathway and TNF signaling pathway.Dayuan Decoction attenuated the expression of TLR4,MyD88 and NF-κB in the perioral skin of the Hp-positive perioral acne rat model,as well as reduced the contents of TNF-α and IL-6 in serum(P<0.01).Conclusion Dayuan Decoction may exert therapeutic effects on Hp-positive perioral acne by modulating the TLR4/NF-κB signaling pathway,reducing the levels of inflammatory factors TNF-α and IL-6,and alleviating the inflammatory damage in the perioral skin.关键词
达原饮/口周痤疮/幽门螺杆菌/网络药理学/TLR4/NF-κB信号通路/炎症Key words
Dayuan Decoction/perioral acne/Helicobacter pylori/network pharmacology/TLR4/NF-κB signaling pathway/inflammation分类
医药卫生引用本文复制引用
夏习林,张平,易园园,刘雪峰..基于网络药理学和动物实验探讨达原饮治疗幽门螺杆菌阳性口周痤疮作用机制[J].中国中医药信息杂志,2024,31(9):41-47,7.基金项目
湖北省自然科学基金创新发展联合基金项目(2024AFD293) （2024AFD293）
