浙江农林大学学报2024，Vol.41Issue(4)：669-678,10.DOI:10.11833/j.issn.2095-0756.20240145
517份玉米种质容重与其他品质性状相关性分析及候选基因挖掘
Correlation analysis of test weight and other quality traits and candidate gene mining in 517 maize germplasms
摘要
Abstract
[Objective]As an important factor of quality traits in national maize standard regional tests,test weight is a complex quantitative trait,and its influencing factor and regulatory mechanism is still unclear.Quality traits such as starch,protein,and fat content are important factors affecting test weight,but the relationship between them is still unclear.[Method]This research determined the test weight,starch,crude protein,gross fat,and amylose content of 517 maize germplasms mainly composed of local maize germplasm resources in Zhejiang Province.On the basis of correlation analysis,the kernels of four germplasms with higher test weight,higher starch content,lower protein content,lower fat content,and four germplasms with opposite phenotypes were selected for RNA-Seq analysis.[Result]By correlation analysis,this research found there was a significant positive correlation between test weight and starch content,while a negative correlation was found between test weight and protein or fat content.RNA-Seq analysis was performed for the extreme germplasm,a total of 159 differentially expressed genes were screened between 2 groups,and 8 candidate genes related to carbon metabolism or amino acid biosynthesis were used for further analysis.The expression difference of these genes was consistent with the change of test weight in different inbred lines by RT-qPCR.[Conclusion]The correlation between test weight and other quality traits was preliminarily clarified,providing superior germplasms and 8 candidate genes that play important roles in carbon reduction,endosperm development,starch metabolism,cellular metabolism,and seed fat accumulation for the selection of new high-quality maize cultivars.[Ch,4 fig.2 tab.40 ref.]关键词
玉米/籽粒容重/品质性状/调控机制Key words
maize/kernel test weight/quality traits/regulation mechanism分类
农业科技引用本文复制引用
杨张钰,陈小央,李燕,李欣泽,白康杰,骆遥,赵光武,施俊生,韩庆辉..517份玉米种质容重与其他品质性状相关性分析及候选基因挖掘[J].浙江农林大学学报,2024,41(4):669-678,10.基金项目
浙江省玉米种质资源精准鉴定评价(2022-2024) （2022-2024）
杭州市科技发展计划项目(202203A02) （202203A02）
国家自然科学基金资助项目(32301833) （32301833）
浙江省自然科学基金探索公益项目(TGN24C130001) （TGN24C130001）
浙江省鲜食玉米育繁推种业创新平台项目(2022-2027) （2022-2027）
浙江农林大学大学生创新创业项目(S202210341085,202210341001) （S202210341085,202210341001）
