浙江农林大学学报2024，Vol.41Issue(4)：724-734,11.DOI:10.11833/j.issn.2095-0756.20240138
暗影饰皮夜蛾线粒体基因组全序列测定与分析
Sequencing and analysis of the complete mitochondrial genome of Garella ruficirra
摘要
Abstract
[Objective]This study,with a comprehensive sequencing and analysis of the mitochondrial genome of Garella ruficirra as well as an investigation into the phylogenetic position of Noctuidae through mitochondrial genome sequencing,is aimed to offer a valuable reference for delving into the phylogenetic relationships and evolutionary processes within the Noctuidae as well as exploring the phylogenetic relationship and evolutionary process of Noctuidae.[Method]First,the Illumina sequencing technique was employed to sequence the complete mitochondrial genome of G.ruficirra,allowing for the analysis of its overall characteristics and base composition.Subsequently,a phylogenetic tree encompassing mitochondrial genomes from 5 genera of Noctuidae,comprising 12 species,was constructed using both maximum likelihood and Bayesian methods so as to facilitate the examination of the phylogenetic relationships within the Noctuidae.[Result]The circular mitochondrial genome of G.ruficirra spanned 15 294 base pairs and encompasses 13 protein synthesis-related genes,22 transfer RNA genes,and two ribosomal RNA genes and its gene arrangement conformed to the standard configuration observed in Lepidoptera mitogenomes,featuring a region rich in adenine and thymine,an A+T content of 80.53％and a significant AT bias.The gene order of trnM-trnI-trnQ in G.ruficirra aligned with other Noctuidae species and the majority of Lepidoptera with ATN sequences serving as initiation codons for all 13 protein-coding genes.While trnSl lacked a DHU arm,the remaining tRNA genes exhibited a typical cloverleaf-shaped structure.According to the study of mitochondrial genome,Garella was most closely related to Nycteola and least closely related to Pseudoips among the 5 genera.[Conclusion]Gene rearrangement was evident in the mitochondrial genome of G.ruficirra.Phylogenetic analyses,indicating that the clustering of G.ruficirra and G.musculana samples was within a single clade.This study has furnished valuable insights into the evolutionary patterns of Garella and offered a foundational framework for investigating the phylogeny and evolution of Noctuidae within the Lepidoptera order.[Ch,4 fig.4 tab.47 ref.]关键词
暗影饰皮夜蛾/线粒体基因组/系统发育关系/基因重排Key words
Garella ruficirra/mitochondrial genome/phylogenetic relationship/gene rearrangement分类
农业科技引用本文复制引用
李妍,舒金平,华克达,张亚波,应玥,张威..暗影饰皮夜蛾线粒体基因组全序列测定与分析[J].浙江农林大学学报,2024,41(4):724-734,11.基金项目
中国林业科学研究院基本科研业务费专项资金项目(CAFYBB2022SY011) （CAFYBB2022SY011）
