中药材2024，Vol.47Issue(2)：281-289,9.DOI:10.13863/j.issn1001-4454.2024.02.003
基于表型性状及SSR标记的天麻种质资源遗传多样性分析
Genetic Diversity Analysis of Gastrodia elata Germplasm Resources Based on Phenotypic Traits and SSR Markers
摘要
Abstract
Objective:To study the genetic diversity of Gastrodia elata,to analyze the differences between the germplasm resources of hybrid and self-crossing.Methods:The 26 kinds of Gastrodia elata germplasm resources were used as materials.Based on the morpho-logical markers,the phenotypic characters of each resource,including plant height,capsule number,inflorescence length,capsule length,capsule width,tuber length,tuber width,single weight and active components contents were analyzed by variance,correlation and clus-ter.Genetic diversity was studied with SSR molecular markers,and SSR fingerprints were constructed.Results:In the phenotypic traits of Gastrodia elata,different traits had different ranges of variation.The traits with larger variation ranges were capsule weight,single weight,tuber length,fresh product yield,dry product yield,gastrodin and hydroxybenzyl alcohol contents.The traits with smaller varia-tion ranges were capsule width,stem thickness,umbilical diameter,number of transverse rings and drying rate.The correlation analysis results showed that the correlation among some characters was significant.The clustering results of phenotypic characters showed that the Gastrodia elata resources were divided into two groups when the threshold value was 11.32.Three pairs of the specific SSR primers were screened from the seven pairs of the primers,whose fingerprint was obtained by PCR amplification.The UPGMA clustering distinguished obviously the inbred lines and the hybrid lines of Gastrodia elata,whose most of the results consistented with phenotypic clustering.Con-clusion:The combination of morphological markers and SSR markers can effectively distinguish the hybrid combinations and self-cross-ing combinations of Gastrodia elata.关键词
天麻/形态学/简单重复序列标记/种质资源/多样性Key words
Gastrodia elata Bl./Morphology/Simple sequence repeat marker/Germplasm resources/Diversity分类
农业科学引用本文复制引用
王彩云,张翔宇,成忠均,武新玲,柳敏,周茂嫦,周雪..基于表型性状及SSR标记的天麻种质资源遗传多样性分析[J].中药材,2024,47(2):281-289,9.基金项目
贵州省科研机构创新能力建设专项(黔科合服企[2020]4012) （黔科合服企[2020]4012）
广州市科技计划项目(201903010008) （201903010008）
贵州省人才基地建设项目(RCJD2020-21) （RCJD2020-21）
毕节市创新平台建设项目(毕科合[2022]10号) （毕科合[2022]10号）
