中药材2024，Vol.47Issue(2)：357-364,8.DOI:10.13863/j.issn1001-4454.2024.02.016
昭通彝良天麻化学成分及其降糖活性研究
Study on the Chemical Constituents and Hypoglycemic Activity of Gastrodia elata in Yiliang County from Zhaotong
摘要
Abstract
Objective:To study the chemical constituents and hypoglycemic activity of Gastrodia elata in Yiliang county from Zhao-tong.Methods:The chemical constituents from the acetone extract of Gastrodia elata in Yiliang county from Zhaotong were isolated and purified by column chromatography with silica gal,Rp-C18 and Sephadex LH-20,the structures were identified by modern spectroscopic techniques.The hypoglycemic activity of the compounds and α-glucosidase inhibitor were studied by using L6 skeletal muscle myotube cells.Results:Twenty-nine compounds were isolated from the acetone extract of Gastrodia elata and identified as β-sitosterol(1),cymbi-nodin-A(2),dipentyl phthalate(3),gastrodin(4),p-hydroxybenzaldehyde(5),4,4'-dihydroxydiphenyl methane(6),p-hydroxybenzyl alcohol(7),β-sitosterol palmitate(8),4,4'-dihydroxydibenzyl ether(9),4-methoxymethyl-phenol(10),ethyl laurate(11),trilinolein(12),ethyl(2E,5S)-5-benzyloxy-2-tetradecenoate(13),n-hexacosane(14),n-tetratriacont-20,23-dienoic acid(15),L-1-methyl citrate(16),di-2-ethylhexyl phthalate(17),adenosine(18),syringate(19),palmitic acid(20),α-D-fructofuranose(21),5-hydroxymethyl-furfural(22),stigmasterol(23),n-hexacos-5,8,11-trienoic acid(24),isomangiferin(25),daucosterol(26),β-sitosterol-3-O-butyl(27),methyl-11-methyl-10-pentadecenoate(28),1-(4-β-D-glucopyranosyloxybenzyl)citrate(29),respectively.Conclusion:Among them,compound 3,8,11 to 13,15,17,21,24,27,28 are isolated from Gastrodia elata for the first time.Compounds 1,3 to 8,10 to 13,17,18,21 show promotion of hypoglycemic activity in L6 myotubular cells,and compounds 11 to 15,20 show inhibitory activity against α-glucosidase.关键词
天麻/化学成分/降糖活性/α-葡萄糖苷酶的抑制活性Key words
Gastrodia elata Bl./Chemical constituents/Hypoglycemic activity/α-Glucosidase inhibitory activity分类
医药卫生引用本文复制引用
闻程志,沈梦霞,李才静,王芳,陈毅坚,李干鹏..昭通彝良天麻化学成分及其降糖活性研究[J].中药材,2024,47(2):357-364,8.基金项目
云南民族大学药用植物功能小分子发现与综合利用省创新团队(2019HC020) （2019HC020）
