中药材2024，Vol.47Issue(2)：437-442,6.DOI:10.13863/j.issn1001-4454.2024.02.029
荜茇酰胺调控STAT3/HIF-1α通路诱导乳腺癌和乳腺细胞凋亡的机制
Effects of Piperlongumine on Apoptosis of Breast Cancer and Breast Cells via STAT3/HIF-1α Pathway
摘要
Abstract
Objective:To investigate the effect of piperlongumine on the proliferation,apoptosis and cell cycle of breast cancer cells MDA-MB-231,MCF-7 and normal breast cells MCF-10A and its potential molecular mechanism.Methods:MDA-MB-231,MCF-7 and MCF-10A cells were treated with different concentrations of piperlongumine,and the cell proliferation was detected by MTT assay.Cell clone formation assay was used to detect cell clone formation ability.Flow cytometry was used to detect cell apoptosis and cell cycle.The protein expressions of cleaved Caspase-3,Bcl-2,Bax,Cyclin D1,p53,p-JAK2,p-STAT3,HIF-1α and Survivin were detected by Western Blot.Results:Piperlongumine inhibited the proliferation and induced apoptosis of MDA-MB-231 and MCF-7 cells in a dose-dependent manner,but had no obvious inhibitory effect on MCF-10A cells.The protein expressions of p53,Bcl-2,Cyclin D1,p-STAT3,Survivin,HIF-1α in MDA-MB-231 cells and p53,p-STAT3,Survivin,HIF-1α in MCF-7 cells were down-regulated and cleaved Caspase-3 and Bax protein expressions in MDA-MB-231 cells were up-regulated.Conclusion:Piperlongumine can inhibit the proliferation and induce apoptosis of breast cancer cells MDA-MB-231 and MCF-7,the mechanism may be related to the negative regulation of STAT3/HIF-1α pathway.关键词
荜茇酰胺/STAT3/HIF-1α/乳腺癌/凋亡Key words
Piperlongumine/STAT3/HIF-1α/Breast cancer/Apoptosis分类
医药卫生引用本文复制引用
陈镝,张藏烨,剡雨彤,汪蕾,郭怡欣,吕莹,张怡荣..荜茇酰胺调控STAT3/HIF-1α通路诱导乳腺癌和乳腺细胞凋亡的机制[J].中药材,2024,47(2):437-442,6.基金项目
陕西省科技发展计划项目(2024JC-YBMS-618) （2024JC-YBMS-618）
西安医学院博士科研启动基金项目(2021DOC05) （2021DOC05）
西安医学院科技能力提升计划项目(2022NLTS025) （2022NLTS025）
