遵义医科大学学报2024，Vol.47Issue(8)：768-774,782,8.
石斛碱对过氧化氢诱导的RGC-5细胞氧化损伤的保护作用
Effect of dendrobine on oxidative stress of RGC-5 induced by hydrogen perox-ide
摘要
Abstract
Objective To study the protective effect of dendrobine on H2O2-induced oxidative damage of optic ganglion cells.Methods The cultured RGC-5 cells were divided into normal control group,H2O2 model group,dendrobine 1 nmol/L group,dendrobine 10 nmol/L group and dendrobine 100 nmol/L group,and the cell via-bility of RGC-5 cells was detected by CCK-8.The content of oxidation and antioxidant substances and the level of ROS in cells were detected by kit method.Western Blot was used to detect the expression of nuclear factor E2-related factor 2(Nrf2)and the downstream target protein.Results Dendrobine at the concentrations of 1,10 and 100 nmol/L has no toxic effect on RGC-5 cell viability.H2O2 significantly reduced the activity of RGC-5 cells from the concentration of 200 μmol/L.Dendrobine at the concentrations of 100 nmol/L can significantly re-duce the leakage rate of LDH,the contents of reactive oxygen species and malondialdehyde in RGC-5 cells in-duced by H2O2,and significantly increase the contents of superoxide dismutase and glutathione.Dendrobine can induce the transfer of Nrf2 from cytoplasm to nucleus in RGC-5 cells,and at the same time positively regulate the protein expression of its downstream antioxidant proteins GPx1,GCLC,NQO 1 and HO-1.Conclusion Dendrob-ine has obvious protective effect on H2O2-induced oxidative damage of RGC-5 cells.Its mechanism may be that Dendrobine promotes nuclear translocation of Nrf2,positively regulates the expression of downstream antioxidant protein on Nrf2 pathway,and thus plays an antioxidant role.关键词
石斛碱/视神经节细胞/过氧化氢/氧化损伤Key words
dendrobine/retinal ganglion cells/hydrogen peroxide/oxidative damage分类
医药卫生引用本文复制引用
毛黔华,许凤青,岳芳,张玮,石京山..石斛碱对过氧化氢诱导的RGC-5细胞氧化损伤的保护作用[J].遵义医科大学学报,2024,47(8):768-774,782,8.基金项目
国家自然科学基金资助项目(NO:81773739 （）
U1812403). （）
