遵义医科大学学报2024，Vol.47Issue(8)：822-828,7.
佛波酯诱导THP-1单核细胞分化为巨噬细胞的条件与机制研究进展
Research progress on the conditions and mechanisms of differentiation of THP-1 monocytes into macrophages induced by phorbol ester
摘要
Abstract
The THP-1 cell line,derived from human leukemic monocytes,is frequently utilized in research to investigate the biological functions and mechanisms of monocytes/macrophages,as well as models of macrophage participation in inflammatory diseases.However,the diversity of experimental techniques for inducing differentia-tion of THP-1 monocytes into macrophages can lead to variability in study outcomes across different laboratories,such as PMA concentration,length of withdrawal period,duration of PMA stimulation,baseline state of cells,cell density,and ambient oxygen tension.Optimizing the PMA-induced THP-1 differentiation scheme can not on-ly facilitate experimental studies,but also reduce the differences in results between laboratories.This review aims to consolidate and analyze the factors influencing differentiation,optimal protocols,and underlying mecha-nisms of PMA-induced differentiation of THP-1 monocytes,so as to provide reference for the research of mono-cyte differentiation into macrophages.关键词
佛波酯/单核细胞/巨噬细胞Key words
Phorbol-12-myristate-13-acetate/monocytes/macrophages分类
医药卫生引用本文复制引用
姜宜秀,罗波,檀军..佛波酯诱导THP-1单核细胞分化为巨噬细胞的条件与机制研究进展[J].遵义医科大学学报,2024,47(8):822-828,7.基金项目
国家自然科学基金资助项目(NO:82360401) （NO:82360401）
国家级大学生创新创业项目(NO:202310661073) （NO:202310661073）
贵州省科技厅计划项目[NO:黔科合基础-ZK(2023)506]. （2023）
