组织工程与重建外科杂志2024，Vol.20Issue(4)：403-410,8.DOI:10.3969/j.issn.1673-0364.2024.04.002
脂肪间充质干细胞经超声刺激后分泌的细胞外囊泡对脂肪间充质干细胞生物学特性的影响
The effect of extracellular vesicles secreted by adipose-derived mesenchymal stem cells after being stimulated by ultrasound on the biological properties of adipose-derived mesenchymal stem cells
摘要
Abstract
Objective To collect extracellular vesicles(EVs)secreted by adipose mesenchymal stem cells(ADSCs)after ultrasound stimulation,compare with EVs secreted by normal ADSCs,and to explore the effects of EVs secreted after ultrasound stimulation on the biological characteristics of ADSCs,including cell proliferation,migration,and adipogenic differentiation abilities.Methods Firstly,some ADSCs were treated with low-intensity ultrasound,cell culture supernatant was collected and extracellular vesicles(U-EVs)were extracted by ultrafiltration method.Some ADSCs were cultured normally and their extracellular vesicles(N-EVs)were also collected.The experiments were performed in the following three groups:PBS(blank control group),EVs secreted by untreated cells(N-EVs group),and EVs secreted by ultrasound-treated cells(U-EVs group).The CCK-8 assay was used to assess the viability of ADSCs under treatment with EVs at different concentrations.The concentration that most strongly enhanced cell viability was selected.At this concentration,cell migration was detected by scratch test,cell proliferation was detected by EdU method,and adipogenic induction medium was used to induce and compare adipogenic differentiation ability of ADSCs.Results EVs secreted after ultrasound stimulation met the identification standards,and the optimal concentration for affecting ADSCs was about 150 μg/mL.At this concentration,the cell proliferation rate of the U-EVs group(19.2%±1.8% )was significantly higher than that of the N-EVs group(11.9%±3.4% ),and the Oil Red O staining area of the U-EVs group was(2.78±0.23)times that of the control group,significantly higher than(2.11±0.18)times of the N-EVs group.However,there was no significant difference in cell migration rates between the N-EVs(16.3%±2.0% )and U-EVs(21.5%±2.3% )groups.Conclusion EVs secreted by ultrasound-stimulated ADSCs significantly enhance the vitality of ADSCs and improve their abilities in cell proliferation,migration,and adipogenic differentiation.关键词
细胞外囊泡/脂肪间充质干细胞/超声Key words
Extracellular vesicles/Adipose-derived mesenchymal stem cell/Ultrasound分类
生物科学引用本文复制引用
刘子博,王一凯,郑毅,潘楚乔,许鹏,刘凯..脂肪间充质干细胞经超声刺激后分泌的细胞外囊泡对脂肪间充质干细胞生物学特性的影响[J].组织工程与重建外科杂志,2024,20(4):403-410,8.基金项目
国家自然科学基金面上项目(82272289) （82272289）
上海市科学技术委员会临床医学研究中心项目(22MC1940300). （22MC1940300）
