EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cottonOACSTPCD
EPSPS is a key gene in the shikimic acid synthesis pathway that has been widely used in breeding crops with herbicide resistance.However,its role in regulating cell elongation is poorly understood.Through the overexpression of EPSPS genes,we generated lines resistant to glyphosate that exhibit an unexpected dwarf phenotype.A representative line,DHR1,exhibits a stable dwarf phenotype throughout its entire growth period.Except for plant height,the other agronomic traits of DHR1 are similar to its transgenic explants ZM24.Paraffin section observations showed that DHR1 internodes are shortened due to reduced elongation and division of the internode cells.Exogenous hormones confirmed that DHR1 is not a classical brassinolide(BR)-or gibberellin(GA)-related dwarfing mutant.Hybridization analysis and fine mapping confirmed that the EPSPS gene is the causal gene for dwarfism,and the phenotype can be inherited in different genotypes.Transcriptome and metabolome analyses showed that genes associated with the phenylpropanoid synthesis pathway are enriched in DHR1 compared with ZM24.Flavonoid metabolites are enriched in DHR1,whereas lignin metabolites are reduced.The enhancement of flavonoids likely results in differential expression of auxin signal pathway genes and alters the auxin response,subsequently affecting cell elongation.This study provides a new strategy for generating dwarfs and will accelerate advancements in light simplification in the cultivation and mechanized harvesting of cotton.
Qingdi Yan;Wei Hu;Chenxu Gao;Lan Yang;Jiaxian Yang;Renju Liu;Masum Billah;Yongjun Lin;Ji Liu;Pengfei Miao;Zhaoen Yang;Fuguang Li;Wenqiang Qin;
State Key Laboratory of Cotton Bio‑breeding and Integrated Utilization/Institute of Cotton Research,Chinese Academy ofAgricultural Sciences,Anyang 455000,China National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research,Huazhong AgriculturalUniversity,Wuhan 430070,ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Zhengzhou University,Zhengzhou 450066,ChinaState Key Laboratory of Cotton Bio‑breeding and Integrated Utilization/Institute of Cotton Research,Chinese Academy ofAgricultural Sciences,Anyang 455000,ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research,Huazhong AgriculturalUniversity,Wuhan 430070,ChinaState Key Laboratory of Cotton Bio‑breeding and Integrated Utilization/Institute of Cotton Research,Chinese Academy ofAgricultural Sciences,Anyang 455000,China National Nanfan Research Institute(Sanya),Chinese Academy of Agricultural Sciences,Sanya 572025,ChinaState Key Laboratory of Cotton Bio‑breeding and Integrated Utilization/Institute of Cotton Research,Chinese Academy ofAgricultural Sciences,Anyang 455000,China State Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Zhengzhou University,Zhengzhou 450066,China
农业科学
EPSPScottonT-DNAdwarfismherbicide resistance
《Journal of Integrative Agriculture》 2024 (010)
P.3437-3456 / 20
supported by funding from the Natural Science Foundation of Henan Province,China(232300421010);the Key Research and Development Project of Henan Province,China(231111110400);the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City,China(320LH045);the Hainan Yazhou Bay Seed Laboratory,China(B21HJ0215);the Fundamental Research Funds of State Key Laboratory of Cotton Biology,China(2021CBE03);the Central Public-interest Scientific Institution Basal Research Fund,China(Y2023XK16);the Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIPIVFCAAS to F.G.L)。
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