重庆理工大学学报2024，Vol.38Issue(15)：281-290,10.DOI:10.3969/j.issn.1674-8425(z).2024.08.033
基于斑马鱼模型研究天麻多糖的免疫调节作用
Assessment of immunomodulatory effect of Gastrodiae rhizoma polysaccharides in zebrafish embryo
摘要
Abstract
Gastrodiae rhizoma polysaccharides (GRP) have garnered attention primarily for their hepatoprotective and anti-cancer properties,with limited exploration in immunomodulation and anti-inflammation.This study,for the first time,evaluates the safety and immune regulatory effects of GRP by using a zebrafish model,elucidating its mechanism of action and furnishing important data for future GRP components research and development.Safety evaluations encompassing survival rate,heart rate,and yolk sac edema of zebrafish are conducted.Additionally,acridine orange,DAF-FM DA,and DCFH-DA fluorescent probes are employed to detect apoptotic cells,nitric oxide (NO) and reactive oxygen species (ROS) levels respectively.The impact of GRP on the expression of immune genes induced by lipopolysaccharide (LPS) is studied through real-time quantitative PCR (RT-qPCR).The effects of GRP on the quantity of macrophages and neutrophils in zebrafish are assessed by using neutral red and Sudan Black B staining.Our resultsshow co-incubation of GRP inhibits the pathological reactions such as increased heart rate,yolk sac edema,and morphological malformations induced by LPS,demonstrating GRP has a protective effect against LPS-induced toxicity in zebrafish embryos.Furthermore,GRP exhibits dese-dependent immunomodulatory effects,suppressing the production of NO and ROS and cell apoptosis induced by LPS.The results of qPCR indicate the anti-inflammatory action of GRP is associated with the downregulation of C3a and TNF-α and the upregulation of IL-10 translation.Moreover,GRP also reduces the accumulation of neutrophils and macrophages at sites of inflammation,highlighting its protective role against LPS-induced effects in zebrafish.关键词
天麻/多糖/免疫调节/斑马鱼/抗炎Key words
Gastrodiae rhizoma/polysaccharides/immunomodulation/zebrafish/anti-inflammatory分类
药学引用本文复制引用
曾易,白钦如,胡瑞雪,王婷,励娜,胡光..基于斑马鱼模型研究天麻多糖的免疫调节作用[J].重庆理工大学学报,2024,38(15):281-290,10.基金项目
重庆市自然科学基金面上项目(CSTB2023NSCQ-MSX0685) （CSTB2023NSCQ-MSX0685）
重庆市中小学创新人才培养工程项目(CY230914) （CY230914）
重庆地方生物医药与大健康产业发展研究智库建设项目 （）
