东南园艺2024，Vol.12Issue(3)：177-182,6.DOI:10.20023/j.cnki.2095-5774.2024.03.003
龙血树组织诱导快速繁殖技术研究
Tissue Culture for Rapid Propagation of Dracaena cambodiana
摘要
Abstract
[Objective]The objective of this study is to explore the effects of different disinfection methods and hormone ratios on rapid propagation technology of Dracaena cambodiana tissue culture,and to provide references for establishing a rapid propagation system of D.cambodiana tissue culture.[Method]The effects of different sterilization times,plant hormone ratios and concentrations on the induction,proliferation,and rooting of adventitious buds were explored using'Space'and'68#'as materials.[Result]The results showed that the aseptic materials could be obtained using the stem with apical bud when it was disinfected by 75%alcohol for 20 seconds and 0.1%mercuric chloride for 8 minutes,achieving the best disinfection effect.The optimal medium for inducing adventitious buds was MS+5.0 mg/L 6-BA+3.0 g/L Huabao;the optimal proliferation medium was MS+2.0 mg/L 6-BA+0.1 mg/L NAA,and the proliferation coefficients of'Space'and'68#'reached 5.35 and 4.87,respectively;the optimal rooting medium was MS+1.0 mg/L NAA and the rooting rates of'Space'and'68#'were both above 99%,with rooting numbers of 9.2 and 11.2,respectively.[Conclusion]The establishment of tissue culture and rapid propagation system of D.cambodiana with apical bud stem as explants will lay a good foundation for the industrial production and genetic transformation of D.cambodiana.关键词
龙血树/植物生长调节剂/组织培养/快速繁殖Key words
Dracaena cambodiana/Plant growth regulator/Tissue culture/Rapid propagation分类
农业科技引用本文复制引用
郑焙华..龙血树组织诱导快速繁殖技术研究[J].东南园艺,2024,12(3):177-182,6.基金项目
福建省林业科技项目(2024FKJ09) （2024FKJ09）
泉州市科技计划项目(2020N026) （2020N026）
