东南园艺2024，Vol.12Issue(4)：271-277,7.DOI:10.20023/j.cnki.2095-5774.2024.04.004
大球盖菇颗粒原种培养基筛选及其应用
Study on Selection and Application of Culture Medium of Particle Pre-culture Spawn of Stropharia rugosoannulata
摘要
Abstract
[Objective]The aim is to screen suitable spawn culture media for Stropharia rugosoannulata and develop particle pre-culture spawn with strong mycelial vitality,numerous germination points,and loose uniformity.The appropriate inoculation amount of spawn using vent plastic bag was established.[Method]Using'S.rugosoannulata No.8'as the experimental strain and the mycelial growth rate as the evaluation index,a single factor experiment was conducted to compare the effects of particle spawn propagation and cultivation on different media containing main components such as millet,fine sawdust,corn cob,and rapeseed meal,and compare the effects of different inoculation amount on the bag-filled time of spawn using vent plastic bag.[Result]The optimal formula for the particle pre-culture spawn medium of S.rugosoannulata was determined to be XGM,with 50%millet,2%sucrose,20%fine sawdust,26%diatomaceous earth,and 2%light calcium carbonate.The carbon-to-nitrogen ratio in the medium was 32.5,and the growth rate of the mycelia of the particle pre-culture spawn was 2.99 mm/d.The optimal inoculation amount was 1.25%and the average bag-filled time was 13.4 days using particle pre-culture spawn XGM to expand spawn in vent plastic bag.[Conclusion]The culture medium of particle pre-culture spawn was optimized and the production process of S.rugosoannulatain using vent plastic bag was preliminarily established,which laid a foundation for industrial production of S.rugosoannulata spawn.关键词
大球盖菇/颗粒原种培养基/透气袋栽培种/优化Key words
Strophariarugosoannulata/Particle pre-culture spawn/Spawn using vent plastic bag/Application分类
农业科技引用本文复制引用
曾志恒,陈文智,戴建清,曾辉,郭仲杰,蔡志欣..大球盖菇颗粒原种培养基筛选及其应用[J].东南园艺,2024,12(4):271-277,7.基金项目
福建省属公益类科研院所基本科研专项(2021R1035002) （2021R1035002）
福建省农业科学院2023年东西部协作项目[闽农科政(2023)8号] （2023）
