东南园艺2024，Vol.12Issue(4)：278-284,7.DOI:10.20023/j.cnki.2095-5774.2024.04.005
红托竹荪液体发酵培养基筛选及培养条件优化
Screening of Liquid Fermentation Medium and culture condition for Dictyophora rubrovolvata
摘要
Abstract
[Objective]Screen the liquid spawn fermentation medium for Dictyophora rubrovolvata and provide references for the application of liquid spawn of D.rubrovolvata in industrialized cultivation.[Method]The effects of medium nutrients and culture methods on liquid fermentation of D.rubrovolvata were investigated with the mycelium biomass as the indicator.[Result]Maltose syrup,corn syrup,corn flour,soybean powder for fermentation and soybean flour are suitable for the liquid fermentation of D.rubrovolvata.When the amount of corn syrup was 20 g/L,the mycelial biomass was 7.13 g/L.Corn flour,soybean powder for fermentation and soybean flour could significantly promote the mycelial biomass in the test range,when the usage was 4 g/L,the mycelial biomass reach the maximum,which were 2.1 g/L,1.8 g/L,and 1.7 g/L respectively.[Conclusion]Using 250 mL Erlenmeyer flasks,the suitable culture conditions for D.rubrovolvata were listed as follows:initial medium pH 5.5,liquid volume 100-120 mL,inoculation volume 16%,and the terminal point of liquid spawn is 14 d.关键词
红托竹荪/液体菌种/培养条件Key words
Dictyophora rubrovolvata/Liquid spawn/Culture method分类
农业科技引用本文复制引用
江晓凌,林辉,杨驰,肖冬来,刘晓瑜,林衍铨,马璐..红托竹荪液体发酵培养基筛选及培养条件优化[J].东南园艺,2024,12(4):278-284,7.基金项目
福建省自然科学基金项目(2021J01504) （2021J01504）
福建省属公益类科研院所基本科研专项(2022R1035005) （2022R1035005）
福建省农业科学院引导性科技创新项目(YDXM202209) （YDXM202209）
福建省农业科学院科技创新团队(CXTD2021016-2) （CXTD2021016-2）
