军事医学2024，Vol.48Issue(8)：601-607,7.DOI:10.7644/j.issn.1674-9960.2024.08.007
Sirt1对创伤弧菌脓毒症中巨噬细胞凋亡的影响及机制
Effects of Sirt1 on macrophage apoptosis in Vibrio vulnificus sepsis
摘要
Abstract
Objective To investigate the role of silencing regulatory protein 1(Sirt1)in the regulation of Vibrio vulnificus sepsis-induced macrophage apoptosis and the molecular mechanisms.Methods Mouse RAW264.7 macrophages which stably overexpressed Sirt1 were constructed and screened by genistein G418.CCK-8 analysis was used to detect the proliferation of cells in the control group and Sirt1-Flag group.The changes of expression levels of apoptosis-associated protein poly ADP-ribose polymerase(PARP),cleaved-PARP,caspase3,cleaved-caspase3 and acetylated p53 in different treatment groups were detected via Western blotting.A Vibrio vulnificus sepsis model in mice was established,and the expression levels of apoptosis-associated protein cleaved-caspase3 in the lung,spleen and liver of mice of different treatment groups were detected by immunohistochemistry.Results Overexpression of Sirt1 reduced VVC-induced RAW264.7 cell damage.Overexpression of Sirt1 as well as RSV pretreatment lowered the expression of apoptosis-associated protein cleaved-PARP,cleaved-caspase3 and acetylated p53 in VVC-stimulated RAW264.7 cells and mouse peritoneal macrophages.In the mouse model of Vibrio vulnificus sepsis,therapeutic administration of RSV reduced the expression of apoptosis-associated protein marker cleaved-caspase3 in lung,spleen and liver tissues.Conclusion Sirt1 can inhibit p53 acetylation and reduces apoptosis in mouse macrophages,which helps protect against Vibrio vulnificus sepsis.关键词
创伤弧菌/白藜芦醇/聚腺苷二磷酸核糖聚合酶/胱天蛋白酶3/沉默调节蛋白1/细胞凋亡/巨噬细胞Key words
Vibrio vulnificus/resveratrol/poly ADP-ribose polymerase/caspase-3/Sirt1/apoptosis/macrophage分类
医药卫生引用本文复制引用
周慧男,吴成林,刘剑飞,张陈,周丽君,钦可为..Sirt1对创伤弧菌脓毒症中巨噬细胞凋亡的影响及机制[J].军事医学,2024,48(8):601-607,7.基金项目
北京市自然科学基金项目(7242135) （7242135）
国家自然科学基金项目(82202382) （82202382）
