军事医学2024，Vol.48Issue(8)：608-613,6.DOI:10.7644/j.issn.1674-9960.2024.08.008
苏拉明改善对乙酰氨基酚诱导小鼠急性肝损伤的作用及机制研究
Effects of suramin on acetaminophen-induced acute liver injury in mice and the mechanism
摘要
Abstract
Objective To investigate the role and mechanism of suramin(Sur)in acetaminophen(APAP)-induced acute liver injury in mice.Methods 8-10 weeks old C57BL/6J mice were randomly divided into the APAP group and APAP+Sur group(20 mg/kg suramin was injected 1 h before).After 18 hrs of fasting,400 mg/kg APAP was injected intraperitoneally to establish a mouse model of acute liver failure and the survival rate was recorded.An acute liver injury model of mice was established via intraperitoneal injection of 300 mg/kg APAP(other conditions remained unchanged).A control group was also established,with liver tissues and serum collected at 0,2,and 12 hours post-APAP treatment.ELISA and CBA techniques were adopted to detect the release of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum and the secretion of inflammatory factors.H&E staining and immunohistochemistry were used to detect liver tissue necrosis and inflammatory cell infiltration.DCFA-DH and ELISA techniques were used to detect the levels of reactive oxygen species(ROS),malondialdehyde(MDA)and glutathione(GSH)in liver tissues.Western blotting was employed to assess the activation of the JNK signaling pathway in liver tissues.Results Suramin treatment improved the survival rate of APAP-induced mice,reduced the release of transaminases and inflammatory factors in serum,and alleviated APAP-induced liver cell necrosis and inflammatory cell infiltration in the liver.Suramin treatment delayed APAP-induced GSH depletion in the liver,reduced MDA and ROS levels,and inhibited JNK pathway activation.Conclusion This study has confirmed the protective effect of suramin against acetaminophen-induced acute liver injury in mice.The mechanism is potentially related to oxidative stress and inflammation.关键词
对乙酰氨基酚/药物性肝损伤/苏拉明/氧化应激/JNK信号通路Key words
acetaminophen/drug-induced liver injury/suramin/oxidative stress/JNK signaling pathway分类
医药卫生引用本文复制引用
陈旭,刘慧茹,王婷,向慎思,詹轶群,杨晓明,任广明..苏拉明改善对乙酰氨基酚诱导小鼠急性肝损伤的作用及机制研究[J].军事医学,2024,48(8):608-613,6.基金项目
北京市自然科学基金项目(7222122) （7222122）
