菌物研究2024，Vol.22Issue(3)：257-267,11.DOI:10.13341/j.jfr.2021.1463
蝙蝠蛾拟青霉Cs-4菌粉有效成分的抗肿瘤作用
Anti-Tumor Function of Active Ingredients from Paecilomyces hepiali CS-4
摘要
Abstract
The present study was conducted to understand the anti-tumor activity and pharmacological mechanisms of effective ingredients extracted from Paecilomyces hepiali Cs-4.By using the mouse H22 cell line of abdominal hepatoma ascites,experimental samples were randomly divided into various groups,each containing ten mice,with or without treatments of five components;polysaccharide,adenosine,chitin,ergosterol and ergosterol peroxide.After ten days of continuous feeding,tumor tissues were taken out and body-mass detection was carried out for the calculating of tumor growth rate and organ index.Cytokine levels of IL-6,IL-2,TNF-α,IFN-γ,VEGF,AST and BUN in the mouse serum,immune responses of VEGF,P53,Bcl-2,Bax and Caspase-3 antibody in the tumor tissues and expression levels of Caspase-3,Bax,Bcl-2 and VEGF proteins were investigated.Status of cell death(apoptosis)was also measured via the TUNEL assay.Our results showed that the combined feeding of five active ingredient components of P.hepiali Cs-4 could generate a positive and multi-way effect on the anti-tumor process through regulation of immune factors in various cells.关键词
抑瘤率/病理切片染色/细胞因子/细胞凋亡/蛋白免疫印迹Key words
anti-tumor rate/pathological slice staining/cytokine/apoptosis/western blot分类
农业科技引用本文复制引用
闫芳,毕建丰,刘楚一,包海鹰,杨明..蝙蝠蛾拟青霉Cs-4菌粉有效成分的抗肿瘤作用[J].菌物研究,2024,22(3):257-267,11.基金项目
国家中药标准化项目(ZYBZH-C-JX-39) （ZYBZH-C-JX-39）
