菌物研究2024，Vol.22Issue(3)：276-282,7.DOI:10.13341/j.jfr.2022.1527
灵芝子实体提取物对C57BL/6小鼠黑色素瘤肺转移的抑制作用
Inhibitory Effect of Ganoderma Fruiting Body Extracts on Lung Metastasis of Melanoma in C57BL/6 Mice
摘要
Abstract
In order to further explore the antitumor activity and its functional mechanism of Ganoderma,a C57BL/6 mouse model of melanoma lung metastasis was established.Drug treatment respectively with the ethanol extracts(EEGL)and polysaccharides(GL-PS)derived from Ganoderma fruiting bodies was performed to the mouse model at doses of 50,100 and 200 mg/kg.After two weeks,lungs of mice in each group were taken out for morphological analysis.Investigation of the pathological changes through HE staining and expression activity of genes related to extracellular matrix degradation and apoptosis in lung tissues through RT-qPCR were also carried out to understand the effects and possible mechanisms of EEGL and GL-PS on metastasis of melanoma in mouse lungs.Our results showed that both of the supply of EEGL and GL-PS could reduce the lung weight and lung index,and the number of metastases in mice as well.The EEGL was found to inhibit the metastasis of melanoma in mouse lungs by up-regulating the gene expression of MMP-2 and MMP-9,and down-regulating the gene expression of TIMP-2.The GL-PS could inhibit the lung metastasis of mouse melanoma,on the other side,by up-regulating the gene expression of Bcl-2 and down-regulating the gene expression of Bax and Caspase-3.The present study thus provides available experiment data for our future research on the bioactivity of Ganoderma and therefore the development of its related products.关键词
灵芝/肿瘤/肺转移/黑色素瘤/凋亡Key words
Ganoderma/tumor/lung metastasis/melanoma/apoptosis分类
医药卫生引用本文复制引用
王芷宁,朱奕囡,谢文琦,张林波..灵芝子实体提取物对C57BL/6小鼠黑色素瘤肺转移的抑制作用[J].菌物研究,2024,22(3):276-282,7.基金项目
贵州省食用菌育种重点实验室开放课题项目(黔科合平台人才[2019]5105-2008号) （黔科合平台人才[2019]5105-2008号）
