基于UPLC-MS/MS羊肚菌子实体生物碱类代谢物差异分析OACSTPCD

In order to investigate and compare the alkaloid compositions between fruiting bodies cultivated with different strains of Morchella,MLM and MTL,methods of UPLC-MS/MS,cluster analysis,sample correlation analysis and orthogonal partial least squares-discriminant analysis were used at the present study.A total of 78 alkaloid metabolites were detected in the fruiting bodies from the MLM and MTL strains of Morchella(77 from MLM and 75 from MTL),which included 34 kinds of alkaloids,six kinds of pyridine alkaloids,two kinds of pyrrole alkaloids,13 kinds of phenolamines,two kinds of quinoline alkaloids,two kinds of piperidine alkaloids,one kind of isoquinoline alkaloids,and 18 kinds of indole alkaloids.A total of 43 metabolites were successfully screened to show a differential change of content in the fruiting bodies between MLM and MTL,in which eight metabolites were upregulated in MLM as compared to MTL.Compared with the MLM,on the other hand,35 metabolites of MTL were downregulated.In MLM,betaine had the highest content but in MTL it was N-(2-hydroxy-4-methoxy phenyl)acetamide.N-p-coumaryl hydroxy-putcine,p-coumaryl choline and n-feruloguidine were found only in MTL while caffeylguidine existed only in MLM.Among the metabolites showing differential changes with a significant difference,salicylamide and 3-hydroxy-2-aminobenzoic acid revealed the highest content in MTL and MLM,respectively,indicating that MLM might have a higher value for nutrition supply and health-care than MTL.Metabolites showing the differential changes between the two strains were mainly distributed within 19 metabolic pathways,including those of biosynthesis pathway of secondary metabolism,tryptophan metabolic pathway,arginine and proline metabolic pathway,and lysine degradation metabolic pathway.The biological characteristics and metabolic pathways analyzed from the alkaloids with differential changes in the fruiting bodies of two different Morchella strains(MLM and MTL)at this study should provide a reference for further development of edible fungi carrying various functions in Morchella.