南京林业大学学报（自然科学版）2024，Vol.48Issue(5)：69-80,12.
红松天然种群遗传多样性分析及核心种质构建
Analysis of genetic diversity and construction of core collections of Korean pine(Pinus koraiensis)natural population
摘要
Abstract
[Objective]Korean pine(Pinus koraiensis)is a valuable tree species that is distributed throughout northeastern China.Over the past century,human interference has led to a gradual decrease in the number of individuals and distribution of its natural population.Assessing the genetic diversity and building a core collection of natural Korean pine could provide a scientific basis for the effective conservation,management,and utilization of Korean pine germplasm resources.[Method]A total of five well-preserved natural populations of Korean pine in Hebei,Wuying,Xiaobeihu and Jixi in Heilongjiang Province and Lushuihe in Jilin Province in northeast China were studied.A combination of phenotypic data and molecular markers was used to construct the core collection.[Result]Molecular and phenotypic ANOVA results showed that the genetic variation of Korean pine natural populations mainly originated from inter-individual differences,which accounted for 96%and 72.84%of the total variation,respectively.The Jixi population was genetically distant from other populations,with an average Fst of 0.026 8.It also had a high genetic diversity,with Shannon and phenotypic diversity index values of 1.111 and 2.00,respectively.The population structure analysis showed that the five Korean pine natural populations had no obvious subpopulation structure.There were no significant changes in the genetic diversity of Korean pine populations among the different forest ages.Additionally,in the younger forest there was no evidence of heterozygous deletions or inbreeding.There was a broad correlation between needle traits and geographic factors,resulting in the phenotypic differentiation of Korean pine populations.[Conclusion]The Shannon and phenotypic diversity indexes of the core collection constructed by combining molecular and phenotypic markers with a 30%sampling ratio were 1.076 and 2.018,respectively,which was representative of the genetic status of Korean pine populations.This information can be used to better manage the germplasm resources of Korean pine and promote its protection and use.The genetic structure characteristics indicated a need to focus on in situ protection of the natural germplasm and to promote ecological recovery,germplasm protection,and use of Korean pine.关键词
红松/天然种群/遗传多样性/遗传结构/核心种质/林木育种/乡土树种Key words
Korean pine(Pinus koreciensis)/natural populations/genetic diversity/genetic structure/core collection/forest breeding/native trees分类
农业科技引用本文复制引用
闫平玉,张磊,王佳兴,冯可乐,王浩浩,张含国..红松天然种群遗传多样性分析及核心种质构建[J].南京林业大学学报（自然科学版）,2024,48(5):69-80,12.基金项目
中央高校基本科研业务费专项资金项目(2572022AW13) （2572022AW13）
林木遗传育种全国重点实验室(东北林业大学)创新项目(2022A02). （东北林业大学）
