南京林业大学学报（自然科学版）2024，Vol.48Issue(5)：123-130,8.
元宝枫嫩枝扦插技术及生根过程的生理生化分析
Softwood cutting technology for Acer truncatum and physiological and biochemical analysis during rooting process
摘要
Abstract
[Objective]In order to investigate the effects of different treatments on the rooting of softwood cuttings of Acer truncatum and find the best treatment method.We analyzed the relationship between changes in nutrient content,oxidase activity,and rooting of cuttings,and preliminarily explored the rooting mechanism of A.truncatum.[Method]Using rejuvenated A.truncatum trees and three-year-old A.truncatum mother trees as study materials,we conducted an analysis of the effects of growth regulators[indole-3-butyric acid(IBA)and 1-naphthaleneacetic acid(NAA)and a rooting solution],cutting positions(upper and lower parts),rejuvenation,and cutting time(June and September)on the rooting of A.truncatum.Furthermore,we examined the rate of root growth,callus formation,number of roots,root length,and other indicators of root development.Additionally,changes in nutrient contents(soluble sugar and soluble protein)and changes in oxidase activity[peroxidase(POD),polyphenol oxidase(PPO),and indole-3-acetic acid oxidase(IAAO)]were measured during the rooting process.[Result]The optimum IBA and NAA concentrations were 500 and 800 mg/L respectively,and the rooting rates were 59.3%and 52.7%,respectively.The optimal concentration for the rooting solution was a 1.43%volume fraction(70-fold)dilution,which achieved a rooting rate of 43.9%.Significant differences were observed among the three treatments,with all rooting indexes of cuttings treated with growth regulators performed better than those of the control.Additionally,the rooting effect of cuttings in June was better than that in September;the rooting effect of rejuvenated mother trees was better than that of 3-year-old trees;and the rooting effect of upper cuttings was better than that of lower cuttings.There were substantial differences observed following these treatments.During the rooting of the cuttings,the changes in the soluble sugar content following the treatment displayed a decreasing trend followed by an increasing trend.The soluble protein content,POD activity,and PPO activity initially increased and then decreased.However,the IAAO activity displayed a trend of decrease-increase-decrease.The control group exhibited a similar changing trend to that of the treatment group,but the peak time for the treatment group was 5-10 days earlier than that of the control.[Conclusion]The adventitious root formation of A.truncatum was categorized as a mixed rooting type.When the upper cuttings of rejuvenated mother trees were treated with IBA 500 mg/L,all rooting indexes were optimized,and the rooting rate peaked at 81.3%.Growth regulator treatment can improve the metabolism rate of nutrients and the synthesis process of related oxidase,and has a facilitative effect on the rooting of cuttings.关键词
元宝枫/生长调节剂/嫩枝扦插/营养物质/氧化酶Key words
Acer truncatum/growth regulator/softwood cutting/nutrient/oxidase分类
农业科技引用本文复制引用
王玉虓,张斌,马秋月,付威,康真,朱长红,李淑娴..元宝枫嫩枝扦插技术及生根过程的生理生化分析[J].南京林业大学学报（自然科学版）,2024,48(5):123-130,8.基金项目
国家自然科学基金项目(32001357,32471929) （32001357,32471929）
江苏省青年基金(面上项目)(BK20211139) （面上项目）
江苏高校优势学科建设工程资助项目(PAPD). （PAPD）
