农业生物技术学报2024，Vol.32Issue(10)：2218-2227,10.DOI:10.3969/j.issn.1674-7968.2024.10.002
水稻粒长基因GW7的分子标记开发与利用
Development and Application of Molecular Marker for Grain Length Gene GW7 in Rice(Oryza sativa)
郑国利 1顾巧美 1杜明 1方玉 2王攀1
作者信息
- 1. 上海中科荃银分子育种技术有限公司,上海 200030
- 2. 上海中科荃银分子育种技术有限公司,上海 200030||安徽荃银高科种业股份有限公司,合肥 230088
- 折叠
摘要
Abstract
Rice(Oryza sativa)grain length is an important agronomic trait,which is closely related to rice yield and appearance quality.In order to further explore new molecular markers for improving rice grain length,this study developed a fluorescent molecular markers of GW7(Gene ID:10208)gene based on the single base mutation of GW7 gene in the functional region,combined with penta-primer amplification refractory mutation system(PARMS).Combined with grain length phenotype and PCR product sequencing,the detection results of the marker in 13 rice parent materials showed that the marker could accurately identify different GW7 genotypes.Then,16 homozygous GW7 genotypes were selected from 94 rice materials by using this marker.The results of marker-assisted selection showed that excellent rice materials with increased grain length could be obtained in F2 generation of'R91'and'zhuo20'.The marker this study developed can help to selecte individual plants with target traits at the seedling stage,and perform cross-breeding and backcrossing during the flowering period,without having to wait until maturity and harvest thereby accelerating the breeding process for rice grain quality traits.关键词
水稻/粒长/五引物扩增受阻突变体系(PARMS)/荧光分子标记/GW7Key words
Rice/Grain length/Penta-primer amplification refractory mutation system(PARMS)/Fluorescent molecular labeling/GW7分类
农业科技引用本文复制引用
郑国利,顾巧美,杜明,方玉,王攀..水稻粒长基因GW7的分子标记开发与利用[J].农业生物技术学报,2024,32(10):2218-2227,10.
