紫薇WOX家族基因鉴定及其对愈伤诱导的影响OA北大核心CSTPCD

As plant specific transcription factors,WOX gene family plays an important role in regulating plant growth and development,tissue and organ genesis and formation.This study used bioinformatics methods to identify 15 members of WOX gene family in the whole genome of Lagerstroemia indica,named LfiWOX1～LfiWOX15,which were distributed on 14 chromosomes.There were large differences in molecular weight and isoelectric point between proteins.All subcellular predictions were located in the nucleus.Systemic evolution analysis showed that genes were classified into 3 categories,and 5 orthologous WOX members closely related to Arabidopsis thaliana and rice(Oryza sativa)WOX genes.Additionally,some WOX genes with specific motifs in L.indica were classified into the same branch on the evolutionary tree,and all family members contain motif 1,motif 2,and motif 5.The WOX family genes contain 9 cis-regulatory elements related to plant growth and development,hormone regulation,and stress,with different members containing different elements.qRT-PCR analysis showed that the expression levels of LfiWOX12,LfiWOX13 and LfiWOX15 in callus tissue increased by 1.5～1.9 times compared to leaves,while the expression levels of other genes in callus tissue decreased by 0.1～90%compared to leaves.It was speculated that LfiWOX12,LfiWOX13 and LfiWOX15 were related to callus formation.The results provide basic data for further studying the regulation of WOX gene on callus induction and theoretical reference for the establishment of efficient regeneration and genetic transformation system in L.indica.