牦牛瘦素基因克隆及其在肾周和皮下脂肪组织的表达和分布OA北大核心CSTPCD

Leptin plays an important role in the regulation of energy balance and fat deposition,but its role in energy metabolism in yaks(Bos grunniens)is not yet clear.This study collected perirenal and subcutaneous brown and white adipose tissues of 1-and 30-day-old healthy male yak calves,cloned the CDS of the leptin gene through RT-PCR,analyzed its sequence characteristics using bioinformatics methods,and detected its expression and distribution in yak perirenal and subcutaneous adipose tissues using qRT-PCR and immunohistochemistry(IHC)staining methods.The results showed that the CDS of yak leptin gene(GenBank No.PP385937)was 504 bp,encoding 167 amino acids,and the homology with wild yak(Bos mutus)reached 99.01%,indicating that the gene was relatively conserved during evolution.The gene expression analysis showed that the leptin expression in the perirenal and subcutaneous adipose tissues of 30-day-old yak were significantly higher than those of 1-day-old yak(P＜0.05).The IHC showed that the leptin positive staining was observed on the cytomembrane of borwn and white adipocytes in perirenal and subcutaneous brown and white adipose tissues,and the leptin positive staining intensity of 30-day-old yak was significantly higher than that of 1-day-old yak(P＜0.05).This study provides basic materials for further elucidating the mechanism of leptin in cold adaptation of yaks.