农业生物技术学报2024，Vol.32Issue(10)：2324-2331,8.DOI:10.3969/j.issn.1674-7968.2024.10.011
安徽白山羊MIR206遗传变异对生长性状和启动子活性的影响
Effects of Genetic Variation of MIR206 on Growth Traits and Promoter Activity of Anhui White Goat(Capra hircus)
摘要
Abstract
MIR206 is a member of the MIR-1 family,which specifically expressed in skeletal muscle of vertebrates.Skeletal muscle is an important part of the body,so MIR206 gene may play an important role in the regulation of animal growth and development.Insertion/Deletion(InDel)is a kind of important genetic variation that may affect gene function by regulating gene expression levels.Therefore,this study investigated the effects of MIR206 InDel variation on growth traits and MIR206 promoter activity of Anhui white goat(Capra hircus)in order to provide theoretical reference for molecular breeding of goat.The body weight,body height,body length,chest circumference,cannon circumference,and rump width data of 431 Anhui white goat were collected.The InDel mutations of MIR206 gene were identified by direct sequencing of PCR products.The genotypes of Anhui white goat were detected by PCR and agarose gel electrophoresis.The distribution of InDel variation in Anhui white goat population was analyzed by calculating genetic parameters.SPSS 25.0 was used to analyze the association between the genotypes and the growth traits of goats.The effect of the genetic variation in the promoter region of MIR206 on promoter activity was analyzed by dual luciferase reporting system.The results of PCR product sequencing and sequence alignment showed that there was a 6 bp InDel mutation(rs653221349)at the 389～394 locus in front of the transcriptional start site of MIR206.There were 3 genotypes of this variant in Anhui white goat population:II(wild genotype),ID(heterozygous genotype)and DD(homozygous deletion genotype),which belonged to moderate polymorphism(0.25＜PIC＜0.5)and was in Hardy-Weinberg imbalance(P＜0.05).The results of association analysis showed that different genotypes of the 6 bp InDel were significantly correlated with body weight,body length and chest circumference of Anhui white goat(P＜0.05).The body weight,body length and chest circumference of ID genotype individuals were significantly better than those of II and DD genotype individuals.Results of the dual luciferase reporting system showed that the 6 bp deletion significantly reduced gene promoter activity.In summary,this study found that there was a 6 bp InDel mutation in the promoter region of MIR206 gene of Anhui white goat,which was significantly associated with growth traits of Anhui white goat and reduced promoter activity,which could be used as a candidate DNA molecular marker for molecular marker-assisted selection of Anhui white goat.关键词
安徽白山羊/生长性状/MIR206基因/插入/缺失(InDel)/启动子活性Key words
Anhui white goat/Growth traits/MIR206gene/Insertion/Deletion(InDel)/Promoter activity分类
农业科技引用本文复制引用
段琴,丁扬,卢嘉妮,张思欢,凌英会..安徽白山羊MIR206遗传变异对生长性状和启动子活性的影响[J].农业生物技术学报,2024,32(10):2324-2331,8.基金项目
国家重点研发计划(2022YFD1300202) （2022YFD1300202）
国家现代肉羊产业技术体系(CARS-38) （CARS-38）
安徽省肉羊良种联合攻关 （）
安徽农业大学大学生创新创业训练计划 （）
