农业生物技术学报2024，Vol.32Issue(10)：2332-2343,12.DOI:10.3969/j.issn.1674-7968.2024.10.012
甲硫氨酸脑啡肽对猪前体脂肪细胞凋亡的调控机理
Regulation Mechanism of Apoptosis of Porcine(Sus scrofa)Precursor Adipocytes by Methionine Enkephalin
摘要
Abstract
Methionine enkephalin(Met-Enk)is an opioid neuropeptide with regulatory effects on cell proliferation and metabolism.In order to investigate the effect of Met-Enk on apoptosis,mitochondrial structure and function of porcine(Sus scrofa)precursor adipocytes,in this study,Met-Enk was used to treat porcine primary precursor adipocytes,and detected apoptosis by using cell counting kit 8(CCK-8)and annexin V/propidium iodide(AV/PI)staining,and observed mitochondrial structural changes by means of transmission electron microscope(TEM)and atomic force microscope(AFM)and detected the changes of mitochondrial function by JC-1 fluorescent probe and other methods.The results showed that Met-Enk treatment resulted in decreased activity and increased apoptosis rate of porcine precursor adipocytes,causing changes in the expression levels of apoptosis markers Caspase 3 and Caspase 9,etc.The TEM results showed that the number of mitochondria in the precursor adipocytes was reduced,the volume was swollen,and mitochondrial cristae were reduced.The AFM results showed that the mitochondria were deformed,and the surface of the membrane appeared to be concave and the roughness was increased;Immunoblot analysis and qPCR revealed that revealed that Met-Enk treatment led to impaired mitochondrial function and promoted apoptotic factors such as Bax/Bak through opioid growth factor receptor(Ogfr),which prompted the release of cytochrome C from mitochondria and promoted apoptosis.This study deeply investigated the mechanism of Met-Enk inducing apoptosis in porcine precursor adipocytes by affecting mitochondrial structure and function,so as to provide a reference for the use of Met-Enk to improve the development of adipose tissues in livestock and poultry.关键词
甲硫氨酸脑啡肽(Met-Enk)/猪/前体脂肪细胞/细胞凋亡/原子力显微镜(AFM)Key words
Methionine enkephalin(Met-Enk)/Porcine/Precursor adipocytes/Apoptosis/Atomic force microscopy(AFM)分类
农业科技引用本文复制引用
陈齐,刘尊海,任泽宇,王文涛,吕睿,王思蒙,孙超..甲硫氨酸脑啡肽对猪前体脂肪细胞凋亡的调控机理[J].农业生物技术学报,2024,32(10):2332-2343,12.基金项目
陕西省重点研发计划(2021-NY-020) （2021-NY-020）
青海省科技计划(2022-QY-219) （2022-QY-219）
