农业生物技术学报2024，Vol.32Issue(10)：2361-2370,10.DOI:10.3969/j.issn.1674-7968.2024.10.014
基于LPS炎症模型探究贝母素甲与贝母素乙对巨噬细胞Raw264.7的抗炎作用
Exploring the Anti-inflammatory Effects of Peimine and Peiminine on Macrophage Raw264.7 Based on LPS Inflammation Model
摘要
Abstract
Peimine and Peiminine are representative alkaloid medicinal component in the lily family herb Fritillaria thunbergii.They play an important role in the anti-inflammatory effect of F.thunbergii.In order to further investigate the anti-inflammatory effects of these 2 types of alkaloids at the cellular level,this study used lipopolysaccharide(LPS)induced Raw264.7 cells as an inflammatory model,Griess,ELISA,Western blot and qRT-PCR were used to analyze the effects of peimine and peiminine on LPS-induced secretion of pro-inflammatory factors nitric oxide(NO),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),IL-1β,nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2)in Raw264.7 cells,the results showed that both peimine and peiminine could inhibit the differentiation of Raw264.7 cells and reduce the pseudopodia produced by LPS stimulation,NO,TNF-α,IL-6 and IL-1β in Raw264.7 cell supernatant of medium and high dose groups of peimine and peiminine significantly decreased(P＜0.05,P＜0.01).Peimine and peiminine inhibited the expression of iNOS and COX-2 protein in Raw264.7 cells,and the inhibition of peiminine on iNOS protein was higher than that of peiminine on COX-2 protein,the inhibitory effect of peiminine a was higher than that of peiminine.The expression of IL-6 and IL-1β in Raw264.7 cells was significantly decreased by both peimine and peiminine.In conclusion,both peimine and peiminine have good anti-inflammatory effect,which provides some data support for the treatment of inflammation by alkaloids.关键词
贝母素甲/贝母素乙/Raw264.7/抗炎活性Key words
Peimine/Peiminine/Raw264/Anti-inflammatory activity分类
农业科技引用本文复制引用
金泽兰,董云哲,李青,董莉莉,郭焕茹,严灿龙,王忠华..基于LPS炎症模型探究贝母素甲与贝母素乙对巨噬细胞Raw264.7的抗炎作用[J].农业生物技术学报,2024,32(10):2361-2370,10.基金项目
浙江省中药材新品种选育重大科技专项(2021C02074) （2021C02074）
宁波市重点研发计划暨"揭榜挂帅"项目(2023Z120) （2023Z120）
