农业生物技术学报2024，Vol.32Issue(10)：2402-2412,11.DOI:10.3969/j.issn.1674-7968.2024.10.018
利用组蛋白去乙酰化酶抑制剂提高猪体细胞核移植胚胎发育率的研究进展
Research Progress on Improvement in Development of Porcine Somatic Cell Nuclear Transfer Embryos by Histone Deacetylase Inhibitors
摘要
Abstract
Somatic cell nuclear transfer(SCNT)has been widely used in protecting endangered species,propagating better varieties and treating diseases.Histone acetylase is an important epigenetic mark regulating the development of early embryo in pig(Sus scrofa),and its correct obliteration and reconstruction are the basis of the embryonic development.With the development of low input next generation sequencing,many studies found that a variety of abnormal epigenetic modification have been found during the reprogramming of somatic cell nuclear transfer embryos.Histone acetylase modification is an important factor that leads to the development block of porcine somatic cell nuclear transfer embryos.In recent years,some small molecule compounds of histone deacetylase inhibitors have been gradually discovered for the improvement of abnormal modification level of histone acetylase during embryonic development.This paper reviews the studies of the effects and mechanisms of histone deacetylase inhibitors on the development of porcine somatic cell nuclear transfer embryos,which provides reference for improving the cloning efficiency in pig.关键词
猪/体细胞核移植(SCNT)/组蛋白去乙酰化酶抑制剂/胚胎/发育Key words
Pig/Somatic cell nuclear transfer(SCNT)/Histone deacetylase inhibitors/Embryo/Development分类
农业科技引用本文复制引用
晏超,陈指龙,彭翠婷,谢浩,张才用,赵玉兰,齐霖,刘永刚,唐中林..利用组蛋白去乙酰化酶抑制剂提高猪体细胞核移植胚胎发育率的研究进展[J].农业生物技术学报,2024,32(10):2402-2412,11.基金项目
广东省基础与应用基础研究基金委员会区域联合基金—青年基金项目(2021A1515110046) （2021A1515110046）
国家自然科学基金青年项目(32302747) （32302747）
深圳市可持续发展科技专项项目(KCXFZ20201221173213037) （KCXFZ20201221173213037）
