日用化学工业（中英文）2024，Vol.54Issue(9)：1030-1038,9.DOI:10.3969/j.issn.2097-2806.2024.09.003
重组人源化胶原蛋白基于体外水平的护肤功效研究
Skin care efficacy study of recombinant humanized collagen based on in vitro level
王建 1范毓慧 1李丹凤 1程宁文 2李玲 1于玉凤3
作者信息
- 1. 山西锦波生物医药股份有限公司 功能蛋白山西省重点实验室,山西 太原 030032
- 2. 山西医科大学,山西 太原 030012
- 3. 山西锦波生物医药股份有限公司 功能蛋白山西省重点实验室,山西 太原 030032||山西医科大学,山西 太原 030012
- 折叠
摘要
Abstract
Studying the skin care efficacy of recombinant humanized collagen based on in vitro level.The stability of the recombinant humanized collagen was first analyzed by treating at different temperatures,then its skincare efficacy based on in vitro level was evaluated by detecting the inhibition rate of elastase,the inhibition rate of collagenase,the protein content of type I collagen in human fibroblasts,the inhibition of reactive oxygen species(ROS)with human keratinocytes,and the effects of the recombinant humanized collagen on the expression of hyaluronic acid(HA),filaggrin(FLG)and transglutaminase 1(TGM1)in keratinocytes.The results showed that recombinant humanized collagen was able to maintain stability at temperatures below 70℃.With regard to its skincare efficacy,recombinant humanized collagen could inhibit elastase and collagenase activities and promote the increase of type I collagen content in human fibroblasts.It also showed good inhibition of ROS in keratinocytes in vitro and could increase the expression of HA,FLG,and TGM1 in keratinocytes.In short,the recombinant humanized collagen exhibited a favourable skin care effect in vitro level.This study proved that it has potential firming,anti-wrinkle,moisturizing,and repairing efficacy,and is a valuable cosmetic raw material.关键词
重组人源化胶原蛋白/稳定性/人成纤维细胞/体外角质形成细胞/护肤功效Key words
recombinant humanized collagen/stability/human fibroblast cell/in vitro keratinocytes/skin care efficacy分类
化学化工引用本文复制引用
王建,范毓慧,李丹凤,程宁文,李玲,于玉凤..重组人源化胶原蛋白基于体外水平的护肤功效研究[J].日用化学工业（中英文）,2024,54(9):1030-1038,9.
