重组弹性蛋白的表征及在紫外诱导皮肤光老化过程中的作用OA北大核心CSTPCD

The recombinant-elastin engineering bacteria was constructed by genetic engineering technology,and then recombinant elastin was obtained by fermentation purification technology.The recombinant elastin was characterized and identified by N-terminal sequencing of amino acids,molecular weight determination,analysis of amino acid composition,protein purity analysis,endotoxicity determination,infrared spectroscopy and circular dichroism analysis.To explore the roles of recombinant elastin in UVB-induced skin photoaging,the effects of recombinant elastin on the adhesion ability and cell viability of L929 mouse embryonic fibroblasts,as well as the effects of recombinant elastin on the expression levels of Collagen Ⅰ,Collagen Ⅲ,Elastin and matrix metalloproteinase 1(MMP-1),were investigated in the model of UVB-induced L929 cell photoaging.The results showed that the secondary structure of recombinant elastin was similar to that of natural elastin.The recombinant elastin showed certain cell viability and no cytotoxicity at 0.1 mg/mL,which significantly promoted the adhesion and proliferation of L929 cells.Additionally,recombinant elastin could also improve the mRNA transcription levels of Collagen Ⅰ,Collagen Ⅲ and Elastin in UVB irradiated cells by down-regulating the expression of MMP-1 in UVB irradiated cells,thus protecting L929 cells from light damage.That was why recombinant elastin could improve the survival rate of photodamaged fibroblasts.