饲料研究2024，Vol.47Issue(16)：40-45,6.DOI:10.13557/j.cnki.issn1002-2813.2024.16.008
胚蛋注射乳酸菌对雏鸡孵化性能和肠道黏膜结构的影响
Effect of embryo egg injection of lactic acid bacteria on hatching performance and intestinal mucosal structure of chicks
摘要
Abstract
The experiment aimed to investigate the effects of injecting lactic acid bacteria into the embryo on the hatching performance and intestinal mucosa structure of chicks.A total of 150 SPF eggs were incubated until 18 days of embryonic age and then randomly divided into two groups,with each group having three replicates,each consisting of 25 eggs.The control group was injected with 0.2 mL of sterilized saline into the amniotic cavity,while the embryonic egg lactic acid bacteria injection group was injected with 0.2 mL containing 1.21×108 CFU/mL of live Lactobacillus salivarius XP132.The experiment spanned from incubation to hatching at 21 days,with a rearing period of 35 days.The results indicated that the injection of lactic acid bacteria into the fertilized eggs significantly increased the body weight of the chick(P＜0.05).Compared to the control group,the embryonic egg lactic acid bacteria injection group showed a significant increase in the thymus index at 7 and 28 days of age,the spleen and bursa of Fabricius indices at 14 days of age,and the thymus and bursa of Fabricius indices at 21 days of age(P＜0.05).Compared to the control group,the villus height(VH)of the duodenum in the embryonic egg lactic acid bacteria injection group was significantly increased(P＜0.05),and the villus to crypt ratio(V/C)was extremely increased(P＜0.01).The crypt depth(CD)of the duodenum at 7 days of age was extremely reduced(P＜0.01),and the CD of the duodenum at 21 and 35 days of age was significantly reduced(P＜0.05).At 7 and 21 days of age,the VH and V/C of the jejunum were significantly increased(P＜0.05),while the CD was significantly decreased(P＜0.05).At 21 days of age,the VH of the ileum was significantly increased(P＜0.05),and the V/C was extremely increased(P＜0.01).At 35 days of age,the VH of the ileum was significantly increased(P＜0.05).Due to the absence of antibiotic use during the rearing process,some chicks in the control group experienced mild bacterial infections.Electron microscopy observation revealed certain damages to the microvilli and mitochondrial structures of the duodenal epithelial cells.However,the application of lactic acid bacteria prevented bacterial infections in the chicks at 7,21,and 35 days of age,and improved the damage to the epithelial cell microvilli,mitochondrial structures,and tight junction proteins.The study shows that the injection of lactic acid bacteria into fertilized eggs can increase the body weight of SPF chicks and has a certain improving effect on their intestinal mucosal structure.关键词
胚蛋注射/鸡胚/乳酸菌/孵化率/体重/免疫器官指数/肠道黏膜结构Key words
embryonic egg injection/chicken embryo/lactic acid bacteria/incubation rate/weight/immune organ index/intestinal mucosal structure分类
农业科技引用本文复制引用
卢佳慧,徐久鹏,李孜恒,范雨欣,常伽翌,王智乐,吕文杰,徐彤..胚蛋注射乳酸菌对雏鸡孵化性能和肠道黏膜结构的影响[J].饲料研究,2024,47(16):40-45,6.基金项目
河北省自然科学基金项目(项目编号:C2023405005) （项目编号:C2023405005）
河北省现代农业产业技术体系肉禽创新团队建设专项资金(项目编号:HBCT2024270205) （项目编号:HBCT2024270205）
河北省自然基金委青年基金(项目编号:C2022405008) （项目编号:C2022405008）
河北省科技厅中央引导地方科技发展资金项目(项目编号:236Z6601G) （项目编号:236Z6601G）
