山西医科大学学报2024，Vol.55Issue(8)：970-975,6.DOI:10.13753/j.issn.1007-6611.2024.08.003
成纤维细胞生长因子2对胎鼠肾小体发育的影响
Effect of fibroblast growth factor 2 on development of renal corpuscles of embryonic mice
摘要
Abstract
Objective To investigate the effect of the fibroblast growth factor 2(FGF2)on the development of in vitro cultured kidney tissues from the 12.5-day embryonic mice,and explore its relationship with the development of renal corpuscle.Methods The kidney tissues of 12.5-day embryonic mice were in vitro cultured with 0,25,100,200,400 ng/mL FGF2 for 2,4,6 d,and then the develop-ment of renal corpuscles was observed by HE staining.Immunohistochemical staining combined with stereo-logical methods were used to observe and semi-quantitatively analyze the expression of FGFR1 in renal corpuscles at different developmental stages.Results The development process of kidney tissues in vitro was similar to the development process in vivo.After cultured with FGF2,the number of renal corpuscles at different stages of development process increased.Immunohistochemical staining and stereo-logical results showed the expression of FGFR1 in renal tissue in vitro cultured with 25,100,200,400 ng/mL FGF2 was significantly higher than that of 0 ng/mL FGF2(P＜0.05),but there was no statistically significant difference among four concentration groups.Conclusion FGF2 may promote the aggregation of mesenchymal cells and the development of renal corpuscles by binding to FGFR1 during the kidney develop-ment process.关键词
成纤维细胞生长因子/成纤维细胞生长因子受体1/小鼠/肾小体/免疫组织化学Key words
fibroblast growth factor/fibroblast growth factor receptor1/mice/renal corpuscle/immunohistochemistry分类
医药卫生引用本文复制引用
薄双玲,孙亚洁,赵欣晨,丁柏伊,乔海兵..成纤维细胞生长因子2对胎鼠肾小体发育的影响[J].山西医科大学学报,2024,55(8):970-975,6.基金项目
吕梁市重点研发计划项目(2020SHFZ43) （2020SHFZ43）
山西医科大学汾阳学院大学生创新创业训练项目(FDC202112) （FDC202112）
