莲心碱通过抑制细胞自噬减轻体外胰腺腺泡细胞急性炎症反应OACSTPCD
Liensinine alleviates acute inflammatory response of pancreatic acinar cells in vitro by inhibiting autophagy
目的 探讨莲心碱(liensinine,LSN)对雨蛙素诱导的急性胰腺炎细胞模型的保护作用及其分子机制.方法 胰腺腺泡细胞AR42J分为对照组、模型组、20 μmol/L LSN组和40 μmol/L LSN组.模型组加入雨蛙素刺激造模;20 μmol/L LSN组和40 μmol/L LSN组加入LSN预处理12 h,再加入雨蛙素刺激造模.采用Western blot检测LC3、Beclin-1和p62蛋白表达水平,采用ELISA法检测炎症因子…查看全部>>
Objective To explore the protective role of liensinine(LSN)in a cellular model of cerulein-induced acute pancreatitis and its underlying mechanism.Methods Pancreatic acinar cells AR42J were divided into control group,model group,20 μmol/L LSN group,and 40 μmol/L LSN group.AR42J cells in model group were stimulated by cerulein,and AR42J cells in 20 μmol/L LSN group and 40 μmol/L LSN group were pretreated with LSN for 12 h followed by stimulation with cerulein…查看全部>>
惠博;张健;李韧;周蕊;杨正安
西安交通大学第二附属医院干四病区,西安 710004西安交通大学第二附属医院干四病区,西安 710004西安交通大学第二附属医院干四病区,西安 710004西安交通大学第二附属医院干四病区,西安 710004西安交通大学第二附属医院干四病区,西安 710004
临床医学
急性胰腺炎胰腺腺泡细胞细胞自噬莲心碱炎症反应细胞损伤细胞凋亡
acute pancreatitispancreatic acinar cellsautophagyliensinineinflammatory responsecell injurycell apoptosis
《山西医科大学学报》 2024 (8)
1001-1007,7
陕西省社会发展科技攻关项目(2016SF-042)
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