中国临床药理学杂志2024，Vol.40Issue(17)：2488-2492,5.DOI:10.13699/j.cnki.1001-6821.2024.17.007
和厚朴酚在乳腺癌细胞上皮间质转化中的作用研究
Effects of honokiol on epithelial mesenchymal transformation of breast cancer cells
摘要
Abstract
Objective To investigate the effect of honokiol on epithelial mesenchymal transformation(EMT)in breast cancer and its mechanism.Methods MDA-MB-231 cells were randomly divided into control group(normal culture),low dose group(40 µmol·L-1 honokiol),middle dose group(50 μmol·L-honokiol),high dose group(60 μmol·L-1 honokiol),si-NC group(transfected with si-NC),si-NF2 group(transfected with si-NF2),DMSO group(treated with dimethyl sulfoxide)and verteporfin group(12 μmol·L-1 Verteporfin,hippo pathway inhibitor).The cell survival rate of each group was detected by methyl thiazolyl tetrozolium(MTT)assay;Transwell assay detected cell migration in each group;Western blot assay was used to detect the expression of EMT-related protein.BALB/c female mice were randomly divided into model group(construct transplanted tumor model),experimental-L group(give 100 mg·kg-1 and honokiol after modeling)and experimental-H group(give 200 mg·kg-1 and honokiol after modeling),with 10 mice in each group.Tumor volume and weight were detected after treatment;Western blot assay was used to detect the expression of EMT-related proteins.Results The cell survival rates of control group,low dose group,middle dose group and high dose group were(100.00±4.02)％,(80.14±6.51)％,(64.05±6.21)％and(53.70±5.19)％;the migration number of cells were 276.30±14.77,219.40±12.50,162.20±9.53 and 100.10±6.50;the relative expression levels of neurofibrin 2(NF2)protein were 0.52±0.04,0.60±0.06,0.65±0.07 and 0.78±0.10;the relative expression levels of programmed cell death ligand 1(PD-L1)protein were 0.70±0.06,0.47±0.04,0.41±0.05 and 0.36±0.03,respectively.The above indexes in low,middle and high dose groups were significantly different from those in control group(all P＜0.05).The tumor volume of model group,experimental-L group and experimental-H group were(922.02±109.61),(743.04±64.04)and(605.00±72.63)mm3;the tumor weight were(0.87±0.10),(0.66±0.10)and(0.53±0.11)g;the relative expression levels of PD-L1 protein were 0.74±0.08,0.51±0.05 and 0.42±0.05;the relative expression levels of N-cadherin protein were 0.75±0.07,0.60±0.10 and 0.51±0.04,respectively.The above indexes in the experimental-L,-H groups were significantly different from those in the model group(all P＜0.05).Conclusion Honokiol may inhibit EMT in breast cancer cells by regulating NF2-hippo signaling pathway in PD-L1.关键词
和厚朴酚/乳腺癌/神经纤维蛋白2-Hippo/Yes相关蛋白1通路/细胞程序性死亡-配体1/上皮间质转化Key words
honokiol/breast cancer/neurofibrin 2-hippo/Yes associated protein 1 pathway/programmed cell death-ligand 1/epithelial mesenchymal transformation分类
医药卫生引用本文复制引用
李谌,王钰..和厚朴酚在乳腺癌细胞上皮间质转化中的作用研究[J].中国临床药理学杂志,2024,40(17):2488-2492,5.基金项目
辽宁省教育厅基金资助项目(LJKZ0807) （LJKZ0807）
锦州医科大学横向课题基金资助项目(2021005) （2021005）
