中国临床药理学杂志2024，Vol.40Issue(17)：2493-2497,5.DOI:10.13699/j.cnki.1001-6821.2024.17.008
微小RNA-454-3p对子宫内膜癌细胞生物学行为的影响
Effect of microRNA-454-3p on biological behavior of endometrial cancer cells
摘要
Abstract
Objective To investigate the effect of microRNA-454-3p(miR-454-3p)on the proliferation,invasion,and migration ability of endometrial cancer(EC)cells and its mechanism.Methods Analyze the expression levels of miR-454-3p in various tissues using the miTED database.Analyze the survival rates of high expression miR-454-3p and low expression miR-454-3p groups in EC patients using the starBase database.Use Targetscan to predict downstream target genes of miR-454-3p.Use the Kaplan Meier Plotter database to analyze the survival rates of the high expression detection of phosphatase and tensin homolog(PTEN)group(average expression level in the high cohort:2 412.45)and the low expression of PTEN(average expression level in the low cohort:733.15)in EC patients.Ishikawa cells were divided into NC-mimics group(transfected with NC-mimics),mimics group(transfected with miR-454-3p mimics),NC-inhibitor group(transfected with NC-inhibitor),and inhibitor group(transfected with miR-454-3p inhibitor).Detect the proliferation ability of cells using cell counting kit-8(CCK-8)assay;evaluate cell invasion and migration abilities using Transwell and scratch experiments,respectively;detection of PTEN RNA expression levels using real-time quantitative polymerase chain reaction(qRT-PCR).Results Compared with normal tissue,the expression level of miR-454-3p in tumor tissue of EC patients increased(1.57 vs 3.26,P＜0.05),and the survival rate of EC patients with low expression of miR-454-3p increased(0.74 vs 0.42,P＜0.05).The cell proliferation ability of the NC mimics group,mimics group,NC inhibitor group and inhibitor group were 3.73±0.02,5.40±0.02,2.06±0.05 and 1.95±0.05;the number of invading cells were 116±17,154±19,855±165 and 447±44;the number of migrating cells were 116±8,154±27,1 518±50 and 1 132±175;the scratch healing rates were(20.00±8.00)％、(39.00±2.00)％、(84.00±1.00)％and(52.00±1.00)％;the expression levels of PTEN mRNA were 1.16±0.03,0.94±0.02,0.85±0.14 and 1.22±0.07,respectively.The differences in the above indicators between the mimics group and the NC mimics group,as well as between the inhibitor group and the NC inhibitor group,were statistically significant(all P＜0.05).The expression level of PTEN in tumor tissue of EC patients decreased(33.45 vs 17.17,P＜0.05),and the survival rate of EC patients with low expression of PTEN increased(41％vs 93％,P＜0.05).Conclusion miR-454-3p may promote the proliferation,invasion,and migration of EC cells by negatively regulating the expression of PTEN.关键词
微小RNA-454-3p/子宫内膜癌/增殖/侵袭/迁移Key words
microRNA-454-3p/endometrial cancer/proliferation/invasion/migration分类
医药卫生引用本文复制引用
潘紫妍,陈雨婷,魏茜茜,王婷,刘杰,胡晨煜,张婷..微小RNA-454-3p对子宫内膜癌细胞生物学行为的影响[J].中国临床药理学杂志,2024,40(17):2493-2497,5.基金项目
国家自然科学基金地区基金资助项目(81960152) （81960152）
石河子大学校级基金资助项目(KX01880307) （KX01880307）
石河子大学校级基金资助项目(KX018904/0305) （KX018904/0305）
