中国临床药理学杂志2024，Vol.40Issue(17)：2508-2512,5.DOI:10.13699/j.cnki.1001-6821.2024.17.011
高糖高脂诱发骨髓间充质干细胞损伤及铁死亡通路信号异常
High glucose and high fat can induce bone marrow mesenchymal stem cells damage and abnormal ferroptosis signaling pathway
摘要
Abstract
Objective To investigate the relationship between the injury and ferroptosis of bone marrow mesenchymal stem cells(BMSCs)induced by high glucose and high fat.Methods BMSCs were divided into normal group(5.50 mmol·L-1 glucose)and high glucose and high fat(HGHF)group(25.00 mmol·L-1 glucose+0.25 mmol·L-1 palmitic acid).Assessment of cellular aging via β-galactosidase staining;enzyme linked immunosorbent assay(ELISA)were used to detect tumor necrosis factor-α(TNF-α),interleukin-10(IL-10)release levels;glutathione(GSH),malondialdehyde(MDA)and ferrous ion(Fe2+)detection kits were used to detect ferroptosis related indicators;Western blotting was used to detect the expression of ferroptosis related signaling pathway protein acyl-CoA synthetase long chain family member 4(ACS14)/arachidonate 15-lipoxygenase(ALOX15)/glutathione peroxidase 4(GPX4).Results The senescence rates of normal group HGHF group were(6.80±1.60)％and(13.00±1.58)％;the levels of TNF-α were(122.54±3.94)and(169.77±2.89)pg·mL-1;the levels of IL-10 were(155.16±3.97)and(105.15±7.30)pg·mL-1;GSH levels were 4.30±0.33 and 1.55±0.14;MDA levels were 2.94±0.10 and 5.84±0.10;Fe2+levels were 6.22±0.35 and 16.13±0.36;the relative expression levels of ACSL4 protein were 0.42±0.05 and 0.84±0.10;the relative ALOX15 protein were 0.61±0.25 and 1.06±0.11;the relative expression levels of GPX4 protein were 1.13±0.17 and 0.33±0.08,respectively.The above indexes in the HGHF group were significantly different from those in the normal group(all P＜0.05).Conclusion 25 mmol·L-1 glucose combined with 0.25 mmol·L-1 palmitic acid for 24 h can be used as a suitable condition to induce BMSCs injury.ferroptosis plays an important role in BMSCs injury induced by high glucose and high fat.关键词
高糖高脂/骨髓间充质干细胞/铁死亡Key words
high glucose and high fat/bone marrow mesenchymal stem cells/ferroptosis分类
医药卫生引用本文复制引用
阿布都拉·米热合买提,祖丽胡马·热合曼,李包娟,叶雨萌,张䶮之..高糖高脂诱发骨髓间充质干细胞损伤及铁死亡通路信号异常[J].中国临床药理学杂志,2024,40(17):2508-2512,5.基金项目
国家自然科学基金资助项目(81760767) （81760767）
新疆天然药物活性组分与释药技术重点实验室基金资助项目(XJDX1713) （XJDX1713）
