中国临床药理学杂志2024，Vol.40Issue(17)：2533-2537,5.DOI:10.13699/j.cnki.1001-6821.2024.17.016
疏肝理脾汤对非酒精性脂肪性肝炎大鼠肠道菌群的影响
Effects of Shugan Lipi decoction on intestinal flora in non-alcoholic steatohepatitis rats
摘要
Abstract
Objective To explore the effect of Shugan Lipi decoction on inflammation and intestinal flora,Toll like receptor 4(TLR4),T cell immunoglobulin domain and mucin domain-3(Tim-3)in non-alcoholic steatohepatitis(NASH)rats.Methods The NASH model was established by feeding methionine and choline deficient diet for 4 weeks.SD rats were randomly divided into blank group(intragastric administration with 0.9％NaCl),model group(NASH model,intragastric administration with 0.9％NaCl),and experimental group(NASH model,intragastric administration with 6.18g·kg-1 Shugan Lipi decoction).Illumina sequencing by synthesis method was used to detect the 16S rRNA sequence of rat Intestinal microbiota.Western blot method was used to detect the expression levels of Tim-3 and TLR4 proteins.Enzyme-linked immunosorbent assay was used to detect tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-10 levels in each group of rats.Results After 4 weeks of medication,the relative abundance of Bacteroidetes in the blank,model and experimental groups were(47.96±10.52)％,(42.90±15.01)％and(57.15±10.99)％;the relative abundance of Firmicutes were(49.27±9.99)％,(53.06±11.47)％and(39.99±11.88)％;the relative expression levels of Tim-3 protein were 1.03±0.07,0.24±0.06 and 1.57±0.11;the relative expression levels of TLR4 protein were 1.04±0.11,3.23±0.33 and 0.94±0.27;the levels of TNF-α were(403.03±25.25),(576.87±60.29)and(385.16±37.67)pg·mL-1;the levels of IL-6 were(125.35±14.07),(189.75±34.30)and(113.71±18.35)pg·mL-1;the levels of IL-10 were(123.20±15.96),(66.71±11.94)and(119.54±10.57)pg·mL-1,respectively.The above indexes in the experimental group showed statistically significant differences compared with the model group(P＜0.01,P＜0.05).Conclusion Shugan Lipi decoction may regulate inflammatory cytokines by affecting intestinal flora and TLR4,Tim-3 protein expression,affect liver inflammatory response,and improve NASH.关键词
疏肝理脾汤/非酒精性脂肪性肝炎/Toll样受体4/T细胞免疫球蛋白黏蛋白-3/肠道菌群/炎症Key words
Shugan Lipi decoction/nonalcoholic steatohepatitis/Toll-like receptor 4/T cell immunoglobulin domain and mucin domain-3/intestinal flora/inflammation分类
中医学引用本文复制引用
石媛媛,王雅,郭丹,钟航宇,郑云杰,张涛..疏肝理脾汤对非酒精性脂肪性肝炎大鼠肠道菌群的影响[J].中国临床药理学杂志,2024,40(17):2533-2537,5.基金项目
国家自然科学基金联合基金资助项目(U21A20413) （U21A20413）
湖南省自然科学基金资助项目(2022JJ30459) （2022JJ30459）
湖南省卫生健康委科研计划基金资助项目(B202303105937) （B202303105937）
湖南省中医药科研计划基金资助项目(B2023080) （B2023080）
