分析化学2024，Vol.52Issue(9)：1328-1336,9.DOI:10.19756/j.issn.0253-3820.241100
基于三脚架DNA探针的电化学发光生物传感器检测外泌体
Tripod DNA Probe-based Electrochemiluminescent Biosensor for Detection of Exosome
摘要
Abstract
The detection of exosomes is of great significance for early diagnosis and treatment of cancer.In this study,based on the π-π interaction of the adenine bases of DNA with graphene,triple-stranded DNA(tsDNA)was assembled on the surface of electrochemically reduced graphene oxide(ERGO)to obtain a tripod DNA probe.The exosomes was specifically captured by exosome membrane protein(CD63)aptamer(apt)sequence at the top of tsDNA,and the introduction of hemin effectively enhanced the signal intensity of the Luminol-hydrogen peroxide(H2O2)electrochemiluminescence(ECL)system.The ECL intensity of the biosensor showed a good linear relationship with logarithm of exosome concentration in the concnetrtion range of 70-1.4×106 particles/μL,and the detection limit was 30 particles/μL(S/N=3).This tripod-structured DNA probe facilitated the capture and detection of larger targets,and was expected to be applied to the analysis of actual clinical samples.关键词
外泌体/三脚架DNA/适配体/生物传感器/电化学发光Key words
Exosomes/Tripod DNA/Aptamer/Biosensors/Electrochemiluminescence引用本文复制引用
贺彰瑾,金鑫,杜雨莹,吴洁宁,鲁理平..基于三脚架DNA探针的电化学发光生物传感器检测外泌体[J].分析化学,2024,52(9):1328-1336,9.基金项目
国家自然科学基金项目(Nos.22276005,21876005)和北京市卓越青年科学家计划项目(No.BJJWZYJH01201910005017)资助. Supported by the National Natural Science Foundation of China(Nos.22276005,21876005)and the Beijing Outstanding Young Scientist Program(No.BJJWZYJH01201910005017). （Nos.22276005,21876005）
