南方农业学报2024，Vol.55Issue(7)：1873-1886,14.DOI:10.3969/j.issn.2095-1191.2024.07.001
珊瑚益生菌筛选及其益生特性分析
Screening of coral probiotics and their probiotic characteristics
摘要
Abstract
[Objective]This study investigated potential probiotics in coral and elucidated their probiotic characteris-tics,thereby contributing novel materials to the coral probiotic resource library and offering theoretical foundation for mi-crobial control research in coral disease management.[Method]Extracellular enzyme producing strains were screened using protease and β-glucosidase activities,antagonistic active strains was screened with Vibrio coralliilyticus as indicator strain,quorum quenching active strains was screened with Chromobacterium violaceum as indicator strain,and strain identification on species and genus was performed via 16S rRNA sequencing.Then,the anti-disease function of probiotics was verified by in-tank addition test,and the probiotic properties of probiotics were predicted by whole genome analysis.[Result]A total of 271 bacterial strains were isolated and purified from 7 coral samples using an in situ simulated cultiva-tion method.Activity screening indicated that 136 strains exhibited protease activity,87 strains exhibited β-glucosidase ac-tivity,20 strains exhibited quorum quenching activity,and 28 strains exhibited antagonistic activity against V.coralliilyti-cus.The bacterial genera capable of producing extracellular enzymes and displaying antagonistic or quorum quenching ac-tivities included Bacillus sp.,Fictibacillus sp.,Exiguobacterium sp.,Ruegeria sp.,Sphingobium sp.,Pseudoalteromo-nas sp.,Vibrio sp.,Micrococcus sp.,Nocardia sp.,and Sphingomonas sp.The in-tank validation test of probiotic disease resistance demonstrated that the Bacillus sp.GXU-Z9 strain could effectively sustain the photosynthetic efficiency and zooxanthellae density of Pocillopora damicornis,thereby protecting the coral from bleaching induced by V.coralliilyti-cus.Whole genome data analysis revealed that Bacillus sp.GXU-Z9 strain possessed highly active amino acid metabolism and transport capabilities,indicating its potential to supply both essential and non-essential amino acids to coral hosts.Ad-ditionally,it could produce compounds beneficial to corals,such as antibiotics,which helped in resisting Vibrio infec-tions,and could supplement the coral's energy requirements by degrading fibrous or protein particulate matter.Concur-rently,its genome encompassed multiple genes associated with amino acid biosynthesis and B Vitamin synthesis,which had the potential to supply essential amino acids and vitamins to the coral hosts,thereby facilitating their energy acquisi-tion.[Conclusion]Bacillus sp.GXU-Z9 strain can effectively sustain the photosynthetic efficiency and zooxanthellae den-sity of P.damicornis,exhibiting remarkable disease resistance.The whole genome of Bacillus sp.GXU-Z9 strain includes genes encoding chitinase and β-glucosidase,enabling the synthesis of essential amino acids and vitamins in substantial amounts.These capabilities assist the coral in digesting carbohydrate enzymes and the provision of amino acids and vita-mins.关键词
珊瑚/益生菌/芽孢杆菌属/拮抗活性/群体淬灭活性/益生特性Key words
corals/probiotics/Bacillus sp./antagonistic activity/quorum quenching/probiotic properties分类
农业科技引用本文复制引用
苏宏飞,刘缘,曾璐佳,王姝颖,黄沁愉,何旭聪..珊瑚益生菌筛选及其益生特性分析[J].南方农业学报,2024,55(7):1873-1886,14.基金项目
国家自然科学基金青年科学基金项目(41706158) （41706158）
广西自然科学基金项目(2023GXNSFAA026388) Youth Science Fund Project of National Natural Science Foundation of China(41706158) （2023GXNSFAA026388）
Guangxi Natural Science Foundation(2023GXNSFAA026388) （2023GXNSFAA026388）
