南方农业学报2024，Vol.55Issue(7)：1907-1915,9.DOI:10.3969/j.issn.2095-1191.2024.07.004
芒果苷抑制脂多糖诱导小鼠肺泡巨噬细胞炎症反应的机制研究
Machenism of mangiferin suppressing the inflammatory response induced by lipopolysaccharide in mouse alveolar macrophage
摘要
Abstract
[Objective]The objective of this study was to analyze the inhibition mechanisms of mangiferin on lipopoly-saccharide(LPS)-induced inflammatory response,with the goal of identifying a potential therapeutic agent for sepsis.[Method]In this research,mouse alveolar macrophage(MH-S)were chosen as the subject of investigation,and the cyto-toxic impact of mangiferin on MH-S was assessed using the CCK-8 assay.According to the cytotoxicity test,the concen-tration of mangiferin was 100 μg/mL.With normal cultured MH-S as the control,10 μg/mL LPS was used for 24 h to con-struct an in vitro inflammatory model(LPS group),and then 100 μg/mL mangiferin was intervened for 24 h(intervention group).The contents of inflammatory factors(IL-1β,TNF-α,IL-6 and IL-18)in MH-S supernatants were measured by ELISA,gene expression of IL-1β,TNF-α,IL-6 and IL-18 was assessed using real-time fluorescence quantitative PCR,and changes in TLR4/NF-κB signaling pathway were measured by Western blotting and immunofluorescence.[Result]Mangiferin did not have obvious toxic effect on MH-S when its concentration was below 287.60 μg/mL.Compared with the control group,the relative expression of IL-1β,TNF-α,IL-6 and IL-18 was extremely significantly increased in the LPS group(P<0.01,the same below),and the contents of IL-1β,TNF-α,IL-6 and IL-18 in the cell supernatant was sig-nificantly(P<0.05,the same below)or extremely significantly increased.Following treatment with mangiferin interven-tion,there was significant decrease in both relative expression levels of IL-1β,TNF-α,IL-6 and IL-18 genes and their se-cretion levels,suggesting that mangiferin could alleviate inflammatory response in MH-S induced by LPS.In addition,relative expression of NF-κB gene and protein expression level in MH-S of LPS group were extremely significantly higher than that of the control group,and the relative expression of NF-κB gene and protein expression level decreased signifi-cantly after mangiferin intervention,indicating that mangiferin could effectively inhibit expression of nuclear transcrip-tion factors in MH-S induced by LPS;the TLR 4 expression level on the surface of MH-S of LPS group was greatly in-creased,but the TLR 4 expression level on MH-S surface decreased greatly after mangiferin intervention,indicating that mangiferin could effectively inhibit TLR 4 expression on the surface of MH-S induced by LPS.[Conclusion]Mangiferin has no obvious inhibitory effect on the proliferation of MH-S cells,and the half proliferation inhibition rate(IC50)of man-giferin is 287.60 μg/mL.Mangiferin has fine protective effect against the LPS-induced inflammatory response in MH-S,and the mechanism of action may be related to the reduction of inflammatory factor secretion by inhibiting the activation of TLR4/NF-κB signaling pathway.关键词
芒果苷/小鼠肺泡巨噬细胞(MH-S)/脂多糖(LPS)/炎症因子/TLR4/NF-κB信号通路Key words
mangiferin/mouse alveolar macrophage(MH-S)/lipopolysaccharide(LPS)/inflammatory factor/TLR4/NF-κB signaling pathway分类
农业科技引用本文复制引用
李亚娟,方秋果,李玉树,孙芹芹,李舜,黄云飞,付强..芒果苷抑制脂多糖诱导小鼠肺泡巨噬细胞炎症反应的机制研究[J].南方农业学报,2024,55(7):1907-1915,9.基金项目
国家自然科学基金项目(31872443) （31872443）
广东省基础与应用基础研究基金项目(2022A1515140052,2023A1515110264) （2022A1515140052,2023A1515110264）
广东省研究生教育创新计划项目(2022JGXM128) National Natural Science Foundation of China(31872443) （2022JGXM128）
Guangdong Basic and Applied Basic Research Project(2022A1515140052,2023A1515110264) （2022A1515140052,2023A1515110264）
Guangdong Postgraduate Education Innovation Plan Project(2022JGXM128) （2022JGXM128）
