南方农业学报2024，Vol.55Issue(7)：1925-1934,10.DOI:10.3969/j.issn.2095-1191.2024.07.006
罗汉果甜苷与黄芪多糖联用对小鼠骨骼肌抗氧化能力的影响
Effects of mogrosides and Astragalus polysacharin combination on antioxidant capacity of mouse skeletal muscle
摘要
Abstract
[Objective]To explore the effect of mogrosides combined with Astragalus polysacharin on improving the antioxidant capacity of skeletal muscle in mice,and to reveal its synergistic mechanism,so as to provide theoretical basis for the development of mogrosides and Astragalus polysacharin as green and safe new feed additives for improving the an-tioxidant capacity of skeletal muscle and meat quality in livestock and poultry.[Method]Forty SPF KM mice were ran-domly divided into four groups:the control(CK)group,the mogrosides(MOG)group,the Astragalus polysacharin(APS)group,and the mogrosides+Astragalus polysacharin combination(MOG+APS)group,with 10 mice in each group.The drug was administered by oral gavage,the mice in the MOG group were given 200 mg/kg mogrosides daily,the mice in the APS group were given 200 mg/kg Astragalus polysacharin daily,the mice in the MOG+APS group were given 200 mg/kg mogrosides+200 mg/kg Astragalus polysacharin daily,and the mice in the CK group were given 0.1 ml of normal saline daily for 4 weeks.After the administration,the organs of mice were collected to calculate the organ in-dex,the antioxidant enzyme activities in serum and skeletal muscle were determined,and the expression changes of antioxidant-related genes and Keap1/Nrf2 signaling pathway-related genes in skeletal muscle of mice were detected by real-time fluorescence quantitative PCR.[Result]Compared with CK group,the average daily feed intake,average daily gain and organ index of MOG group,APS group and MOG+APS group had no significant changes(P>0.05).The con-tent of malondialdehyde(MDA)in serum and gastrocnemius muscle of mice in MOG group,APS group and MOG+APS group was significantly decreased compared to that in CK group(P<0.05,the same below),while the activities of glutathi-one peroxidase(GSH-Px)and superoxide dismutase(SOD)were significantly increased.The content of MDA in serum and gastrocnemius muscle of mice in MOG+APS group was significantly decreased compared to that in MOG group and APS group,while the activities of GSH-Px and SOD were significantly increased.Compared with the MOG group and APS group,the relative expression of GSH-Px and SOD genes in the gastrocnemius muscle of MOG+APS group showed significant upward trend,the relative expression of NF-E2-related factor 2 gene(Nrf2)was significantly increased by 19.02%and 20.10%respectively,while the relative expression of Kelch-like epichlorohydrin-related protein 1 gene(Keap1)was significantly decreased by 29.92%and 24.20%respectively.[Conclusion]Mogrosides and Astragalus poly-sacharin combination can activate Keap1/Nrf2 signaling pathway,up-regulate SOD and GSH-Px genes expression,in-crease antioxidant enzyme activity,and enhance the antioxidant capacity of skeletal muscle in mice.It is indicated that mogrosides and Astragalus polysacharin can be used as green and safe new feed additives to improve the antioxidant ca-pacity of skeletal muscle and meat quality of livestock and poultry.关键词
小鼠/罗汉果甜苷/黄芪多糖/骨骼肌/抗氧化能力/Keap1/Nrf2信号通路Key words
mice/mogrosides/Astragalus polysacharin/skeletal muscle/antioxidant capacity/Keap1/Nrf2 signa-ling pathway分类
农业科技引用本文复制引用
卢晓凤,骆隽言,韦明涵,农彩密,党钊林,李银,王倩,蒋钦杨,黄艳娜..罗汉果甜苷与黄芪多糖联用对小鼠骨骼肌抗氧化能力的影响[J].南方农业学报,2024,55(7):1925-1934,10.基金项目
国家自然科学基金项目(32360839,31760672) （32360839,31760672）
国家级大学生创新创业训练计划项目(202310593044) National Nature Science Foundation of China(32360839,31760672) （202310593044）
National College Stu-dents'Innovation and Entrepreneurship Training Project(202310593044) （202310593044）
