南方农业学报2024，Vol.55Issue(7)：1949-1960,12.DOI:10.3969/j.issn.2095-1191.2024.07.008
罗汉果甜苷Ⅲ延缓猪卵母细胞体外老化的作用机制
Mechanism effects of mogroside Ⅲ on the alleviation of porcine oocyte aging
摘要
Abstract
[Objective]The study aimed to verify whether mogroside Ⅲ had the effects to delay the aging process of porcine oocytes in vitro and provide reference for utilizing plant extracts to alleviate oocyte aging in vitro.[Method]Por-cine oocytes cultured in vitro for 44 h were used as research subjects.The effective concentration of mogroside Ⅲ for por-cine oocytes was screened by parthenogenetic activation(PA).Three treatment groups were set up:fresh oocytes(Fresh),aging oocytes(Aging),and aging oocytes supplemented with mogroside Ⅲ.Immunofluorescence staining and real-time fluorescence quantitative PCR were employed to detect mitochondrial content and distribution,adenosine tri-phosphate(ATP)content,mitochondrial membrane potential,reactive oxygen species(ROS)levels,DNA damage,antioxidant-related gene expression,and early apoptosis levels in porcine oocytes.[Result]Aging extremely significantly decreased the embryonic cleavage rate and blastocyst development rate of parthenogenetic activated embryos derived from porcine oocytes(P<0.01,the same below).The addition of mogroside Ⅲ effectively improved the embryonic cleavage rate and blastocyst development rate of aging oocytes,especially at 25.0 µmol/L.which was therefore determined as the effective concentration for mogroside Ⅲ.Compared with the Fresh group,the Aging group exhibited significant de-crease in mitochondrial fluorescence intensity(P<0.05,the same below),and extremely significant decrease in ATP con-tent,significant decrease in mitochondrial membrane potential,and extremely significant decrease in the relative expres-sion levels of antioxidant-related genes,while ROS levels,γ-H2AX fluorescence intensity,and Annexin-V positive rate were extremely significantly increased.However,the addition of 25.0 µmol/L mogroside Ⅲ effectively improved the above problems,indicating that mogroside Ⅲ could effectively alleviate the aging process of porcine oocytes.[Conclu-sion]Mogroside Ⅲ effectively delays the aging process of porcine oocytes by improving mitochondrial number and ATP content,restoring ROS balance,up-regulating the expression of antioxidant-related genes,and alleviating DNA damage and early apoptosis caused by the aging of porcine oocytes,thereby enhancing the in vitro developmental competence of porcine oocytes.关键词
猪/卵母细胞/老化/罗汉果甜苷Ⅲ/氧化应激Key words
pigs/oocytes/aging/mogroside Ⅲ/oxidative stress分类
农业科技引用本文复制引用
赵磊,曾筱乔,高祖维,吴诗瑶,彭珂,严煜钧,万润田,梁兴伟..罗汉果甜苷Ⅲ延缓猪卵母细胞体外老化的作用机制[J].南方农业学报,2024,55(7):1949-1960,12.基金项目
国家自然科学基金面上项目(32372881) （32372881）
国家自然科学基金地区科学基金项目(82160287) General Project of National Natural Science Foundation of China(32372881) （82160287）
Regional Project of National Natural Science Foundation of China(82160287) （82160287）
